General method for direct cloning of DNA fragments generated by the polymerase chain reaction

A general method is described for direct cloning of DNA fragments generated by primed enzymatic amplification that is based on digestibility of the cloning vector with XcmI restriction endonuclease to yield a unit linear molecule with 3' unpaired deoxythymidine residues at both ends. Such vecto...

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Veröffentlicht in:	Nucleic acids research 1991-08, Vol.19 (16), p.4560-4560
Hauptverfasser:	KOVALIC, D, JIN-HWAN KWAK, WEISBLUM, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular - methods DNA - metabolism DNA-Directed DNA Polymerase - metabolism Endodeoxyribonucleases - metabolism Fundamental and applied biological sciences. Psychology genes Genetic engineering Genetic technics Genetic Vectors - genetics Methods. Procedures. Technologies Molecular cloning Molecular Sequence Data plasmids Polymerase Chain Reaction Taq Polymerase
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Beschreibung
Zusammenfassung:	A general method is described for direct cloning of DNA fragments generated by primed enzymatic amplification that is based on digestibility of the cloning vector with XcmI restriction endonuclease to yield a unit linear molecule with 3' unpaired deoxythymidine residues at both ends. Such vectors are capable of efficient ligation with products that are obtained by primed enzymatic amplification with Taq DNA polymerase bearing 3' unpaired deoxyadenosine residues at both ends.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/19.16.4560