A cDNA clone from Zea mays endosperm sucrose synthetase mRNA

A cDNA clone from Zea mays endosperm sucrose synthetase mRNA

A cDNA clone for maize endosperm sucrose synthetase of 620 nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoted translation. In Southern blotting to genomic BamHI-di...

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Veröffentlicht in:Nucleic acids research 1980-12, Vol.8 (24), p.6175-6188
Hauptverfasser: Geiser, Martin, Döring, Hans-Peter, Wöstemeyer, Johannes, Behrens, Ute, Tillmann, Edith, Starlinger, Peter
Format: Artikel
Sprache:eng
Schlagworte:
Base Composition
Cloning, Molecular
DNA - isolation & purification
DNA - metabolism
DNA Restriction Enzymes
Escherichia coli - genetics
Fructose - genetics
Glucosyltransferases - genetics
Nucleic Acid Hybridization
Plants - genetics
Plasmids
Protein Biosynthesis
RNA, Messenger - metabolism
Zea mays
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Zusammenfassung:A cDNA clone for maize endosperm sucrose synthetase of 620 nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoted translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of ∼20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/8.24.6175