Site specific endonuclease from Fusobacterium nucleatum

Site specific endonuclease from Fusobacterium nucleatum

Four different isolates of Fusobacterium nucleatum (A,C, D and E) contain restriction endonucleases of differing specificity. Whilst many of the endonucleases are isochizomers of known enzymes, two novel activities are Fnu DII which recognizes and cleaves the sequence5'-CG↓ CG-3'3'-GC...

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Veröffentlicht in:Nucleic acids research 1979-01, Vol.6 (1), p.1-15
Hauptverfasser: Lui, A.C.P., McBride, B.C., Vovis, G.F., Smith, M.
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
DNA Restriction Enzymes - isolation & purification
DNA Restriction Enzymes - metabolism
Fusobacterium - enzymology
Fusobacterium nucleatum
Molecular Weight
Oligodeoxyribonucleotides - analysis
Substrate Specificity
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Beschreibung
Zusammenfassung:Four different isolates of Fusobacterium nucleatum (A,C, D and E) contain restriction endonucleases of differing specificity. Whilst many of the endonucleases are isochizomers of known enzymes, two novel activities are Fnu DII which recognizes and cleaves the sequence5'-CG↓ CG-3'3'-GC↑ GC-5' and Fnu El which recognizes and cleaves the sequence 5'-GA↓ TC-3'3'-CT↑ AG-5' irrespective of the extent of methylation of the adenine residues.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/6.1.1