Angiopoeitin-2 modulates Survivin expression in OxLDL-induced endothelial cell apoptosis
► OxLDL down-regulated the expression of Ang-2 and Survivin. ► Survivin is a target gene of Ang-2. ► Ang-2 attenuates the OxLDL-mediated down-regulation of Survivin. ► Overexpression of Survivin attenuated OxLDL-induced HAEC apoptosis. Angiopoeitin-2 (Ang-2) antagonizes Angiopeitin-1 (Ang-1)-mediate...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2012-01, Vol.417 (1), p.619-622 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Li, Rongsong Mittelstein, David Fang, Karen Beebe, Tyler Quigley, Katherine Berliner, Judith Hsiai, Tzung K. |
| description | ► OxLDL down-regulated the expression of Ang-2 and Survivin. ► Survivin is a target gene of Ang-2. ► Ang-2 attenuates the OxLDL-mediated down-regulation of Survivin. ► Overexpression of Survivin attenuated OxLDL-induced HAEC apoptosis.
Angiopoeitin-2 (Ang-2) antagonizes Angiopeitin-1 (Ang-1)-mediated Tie-2 signaling. Ang-1 is reported to up-regulate anti-apoptotic Survivin expression. Here, we investigated the interplay between Ang-2 and Survivin in response to oxidized low density lipoprotein (OxLDL)-induced apoptosis. We demonstrate that treatment of human aortic endothelial cells (HAEC) with 100μg/ml of OxLDL down-regulated Ang-2 expression as early as 4h after treatment and persisted up to 24h (p |
| doi_str_mv | 10.1016/j.bbrc.2011.12.018 |
| format | Article |
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Angiopoeitin-2 (Ang-2) antagonizes Angiopeitin-1 (Ang-1)-mediated Tie-2 signaling. Ang-1 is reported to up-regulate anti-apoptotic Survivin expression. Here, we investigated the interplay between Ang-2 and Survivin in response to oxidized low density lipoprotein (OxLDL)-induced apoptosis. We demonstrate that treatment of human aortic endothelial cells (HAEC) with 100μg/ml of OxLDL down-regulated Ang-2 expression as early as 4h after treatment and persisted up to 24h (p<0.05, n=3), but did not down-regulate Survivin until the 24h point. Further, treatment of HAEC with recombinant Ang-2 up-regulated Survivin expression (at Ang-2 ⩾200ng/ml, p<0.05, n=3) and attenuated the OxLDL-mediated down-regulation of Survivin (p<0.05, n=3). Knockdown of Ang-2 further down-regulated Survivin expression, whereas over-expression of Survivin attenuated OxLDL-induced HAEC apoptosis (p<0.05, n=3). Hence, Ang-2 mediated Survivin expression in response to OxLDL-induced endothelial apoptosis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2011.12.018</identifier><identifier>PMID: 22182412</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Angiopoietin-2 (Ang-2) ; Angiopoietin-2 - genetics ; Angiopoietin-2 - metabolism ; Apoptosis ; Atherosclerosis ; Cells, Cultured ; Endothelial cells ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Endothelium, Vascular - physiology ; Gene Expression Regulation ; Gene Knockdown Techniques ; Humans ; Inhibitor of apoptosis protein (IAP) ; Inhibitor of Apoptosis Proteins - genetics ; Lipoproteins, LDL - metabolism ; Lipoproteins, LDL - pharmacology ; low density lipoprotein ; Oxidized low density lipoprotein (OxLDL) ; Survivin</subject><ispartof>Biochemical and biophysical research communications, 2012-01, Vol.417 (1), p.619-622</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><rights>2011 Elsevier Inc. All rights reserved 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-17667d44a2367178516cf60e258c7399b982dbab17cacabdb2227fec5201ab7a3</citedby><cites>FETCH-LOGICAL-c487t-17667d44a2367178516cf60e258c7399b982dbab17cacabdb2227fec5201ab7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X11022200$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22182412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Rongsong</creatorcontrib><creatorcontrib>Mittelstein, David</creatorcontrib><creatorcontrib>Fang, Karen</creatorcontrib><creatorcontrib>Beebe, Tyler</creatorcontrib><creatorcontrib>Quigley, Katherine</creatorcontrib><creatorcontrib>Berliner, Judith</creatorcontrib><creatorcontrib>Hsiai, Tzung K.</creatorcontrib><title>Angiopoeitin-2 modulates Survivin expression in OxLDL-induced endothelial cell apoptosis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► OxLDL down-regulated the expression of Ang-2 and Survivin. ► Survivin is a target gene of Ang-2. ► Ang-2 attenuates the OxLDL-mediated down-regulation of Survivin. ► Overexpression of Survivin attenuated OxLDL-induced HAEC apoptosis.
Angiopoeitin-2 (Ang-2) antagonizes Angiopeitin-1 (Ang-1)-mediated Tie-2 signaling. Ang-1 is reported to up-regulate anti-apoptotic Survivin expression. Here, we investigated the interplay between Ang-2 and Survivin in response to oxidized low density lipoprotein (OxLDL)-induced apoptosis. We demonstrate that treatment of human aortic endothelial cells (HAEC) with 100μg/ml of OxLDL down-regulated Ang-2 expression as early as 4h after treatment and persisted up to 24h (p<0.05, n=3), but did not down-regulate Survivin until the 24h point. Further, treatment of HAEC with recombinant Ang-2 up-regulated Survivin expression (at Ang-2 ⩾200ng/ml, p<0.05, n=3) and attenuated the OxLDL-mediated down-regulation of Survivin (p<0.05, n=3). Knockdown of Ang-2 further down-regulated Survivin expression, whereas over-expression of Survivin attenuated OxLDL-induced HAEC apoptosis (p<0.05, n=3). Hence, Ang-2 mediated Survivin expression in response to OxLDL-induced endothelial apoptosis.</description><subject>Angiopoietin-2 (Ang-2)</subject><subject>Angiopoietin-2 - genetics</subject><subject>Angiopoietin-2 - metabolism</subject><subject>Apoptosis</subject><subject>Atherosclerosis</subject><subject>Cells, Cultured</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endothelium, Vascular - physiology</subject><subject>Gene Expression Regulation</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Inhibitor of apoptosis protein (IAP)</subject><subject>Inhibitor of Apoptosis Proteins - genetics</subject><subject>Lipoproteins, LDL - metabolism</subject><subject>Lipoproteins, LDL - pharmacology</subject><subject>low density lipoprotein</subject><subject>Oxidized low density lipoprotein (OxLDL)</subject><subject>Survivin</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhBTig3OglwTNJ7ERCSFWhgLRSD4DUm2U7s61XWTvYyaq8PV5tW8GlJ8vyN7_8z8fYW-AVcBAftpUx0VbIASrAikP3jK2A97xE4M1ztuKcixJ7uD5hr1La8gw2on_JThChwwZwxa7P_Y0LUyA3O19isQvDMuqZUvFjiXu3d76guylSSi74It-u7taf16Xzw2JpKMgPYb6l0emxsDSOhZ7CNIfk0mv2YqPHRG_uz1P26_LLz4tv5frq6_eL83Vpm07OJUgh5NA0GmshQXYtCLsRnLDtrKz73vQdDkYbkFZbbQaDiHJDts2ttZG6PmWfjrnTYnY0WPJz1KOaotvp-EcF7dT_L97dqpuwVzUKzjvMAe_vA2L4vVCa1c6lQxftKSxJ9SBalA1vM3n2JAmyrtu6lm2TUTyiNoaUIm0ePwRcHeSprTrIUwd5ClBleXno3b9VHkcebGXg4xGgvNC9o6iSdeSzCBfJzmoI7qn8v9fDrJY</recordid><startdate>20120106</startdate><enddate>20120106</enddate><creator>Li, Rongsong</creator><creator>Mittelstein, David</creator><creator>Fang, Karen</creator><creator>Beebe, Tyler</creator><creator>Quigley, Katherine</creator><creator>Berliner, Judith</creator><creator>Hsiai, Tzung K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120106</creationdate><title>Angiopoeitin-2 modulates Survivin expression in OxLDL-induced endothelial cell apoptosis</title><author>Li, Rongsong ; Mittelstein, David ; Fang, Karen ; Beebe, Tyler ; Quigley, Katherine ; Berliner, Judith ; Hsiai, Tzung K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-17667d44a2367178516cf60e258c7399b982dbab17cacabdb2227fec5201ab7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Angiopoietin-2 (Ang-2)</topic><topic>Angiopoietin-2 - genetics</topic><topic>Angiopoietin-2 - metabolism</topic><topic>Apoptosis</topic><topic>Atherosclerosis</topic><topic>Cells, Cultured</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endothelium, Vascular - physiology</topic><topic>Gene Expression Regulation</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Inhibitor of apoptosis protein (IAP)</topic><topic>Inhibitor of Apoptosis Proteins - genetics</topic><topic>Lipoproteins, LDL - metabolism</topic><topic>Lipoproteins, LDL - pharmacology</topic><topic>low density lipoprotein</topic><topic>Oxidized low density lipoprotein (OxLDL)</topic><topic>Survivin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Rongsong</creatorcontrib><creatorcontrib>Mittelstein, David</creatorcontrib><creatorcontrib>Fang, Karen</creatorcontrib><creatorcontrib>Beebe, Tyler</creatorcontrib><creatorcontrib>Quigley, Katherine</creatorcontrib><creatorcontrib>Berliner, Judith</creatorcontrib><creatorcontrib>Hsiai, Tzung K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Rongsong</au><au>Mittelstein, David</au><au>Fang, Karen</au><au>Beebe, Tyler</au><au>Quigley, Katherine</au><au>Berliner, Judith</au><au>Hsiai, Tzung K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiopoeitin-2 modulates Survivin expression in OxLDL-induced endothelial cell apoptosis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2012-01-06</date><risdate>2012</risdate><volume>417</volume><issue>1</issue><spage>619</spage><epage>622</epage><pages>619-622</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► OxLDL down-regulated the expression of Ang-2 and Survivin. ► Survivin is a target gene of Ang-2. ► Ang-2 attenuates the OxLDL-mediated down-regulation of Survivin. ► Overexpression of Survivin attenuated OxLDL-induced HAEC apoptosis.
Angiopoeitin-2 (Ang-2) antagonizes Angiopeitin-1 (Ang-1)-mediated Tie-2 signaling. Ang-1 is reported to up-regulate anti-apoptotic Survivin expression. Here, we investigated the interplay between Ang-2 and Survivin in response to oxidized low density lipoprotein (OxLDL)-induced apoptosis. We demonstrate that treatment of human aortic endothelial cells (HAEC) with 100μg/ml of OxLDL down-regulated Ang-2 expression as early as 4h after treatment and persisted up to 24h (p<0.05, n=3), but did not down-regulate Survivin until the 24h point. Further, treatment of HAEC with recombinant Ang-2 up-regulated Survivin expression (at Ang-2 ⩾200ng/ml, p<0.05, n=3) and attenuated the OxLDL-mediated down-regulation of Survivin (p<0.05, n=3). Knockdown of Ang-2 further down-regulated Survivin expression, whereas over-expression of Survivin attenuated OxLDL-induced HAEC apoptosis (p<0.05, n=3). Hence, Ang-2 mediated Survivin expression in response to OxLDL-induced endothelial apoptosis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22182412</pmid><doi>10.1016/j.bbrc.2011.12.018</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2012-01, Vol.417 (1), p.619-622 |
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| subjects | Angiopoietin-2 (Ang-2) Angiopoietin-2 - genetics Angiopoietin-2 - metabolism Apoptosis Atherosclerosis Cells, Cultured Endothelial cells Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Endothelium, Vascular - physiology Gene Expression Regulation Gene Knockdown Techniques Humans Inhibitor of apoptosis protein (IAP) Inhibitor of Apoptosis Proteins - genetics Lipoproteins, LDL - metabolism Lipoproteins, LDL - pharmacology low density lipoprotein Oxidized low density lipoprotein (OxLDL) Survivin |
| title | Angiopoeitin-2 modulates Survivin expression in OxLDL-induced endothelial cell apoptosis |
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