Differential expression of interleukin-32 in chronic rhinosinusitis with and without nasal polyps

To cite this article: Keswani A, Chustz RT, Suh L, Carter R, Peters AT, Tan BK, Chandra R, Kim S‐H, Azam T, Dinarello CA, Kern RC, Schleimer RP, Kato A. Differential expression of interleukin‐32 in chronic rhinosinusitis with and without nasal polyps. Allergy 2012; 67: 25–32. Background: Chronic rhinosinusitis (CRS) is a heterogeneous disease characterized by local inflammation of the upper airways and sinuses and is frequently divided into polypoid CRS (CRSwNP) and nonpolypoid CRS (CRSsNP). However, the mechanism of inflammation in CRS has still not been fully elucidated. The aim of the study was to investigate the role of interleukin‐32 (IL‐32), a recently discovered proinflammatory cytokine, in CRS. Methods: We collected nasal epithelial cells and nasal tissue from patients with CRS and control subjects. We assayed mRNA for IL‐32 by real‐time PCR and measured IL‐32 protein using ELISA, Western blot, and immunohistochemistry. Results: The expression of mRNA for IL‐32 was elevated in epithelial cells from uncinate tissue from patients with CRSsNP compared with patients with CRSwNP (P