Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors
BACKGROUND: Reports of Monosomy 7 in patients receiving granulocyte–colony‐stimulating factor (G‐CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G‐CSF produces Monosomy 7 aneuploidy in healthy d...
Gespeichert in:
| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2012-03, Vol.52 (3), p.537-541 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 541 |
|---|---|
| container_issue | 3 |
| container_start_page | 537 |
| container_title | Transfusion (Philadelphia, Pa.) |
| container_volume | 52 |
| creator | Olnes, Matthew J. Poon, Andrea Miranda, Susan J. Pfannes, Loretta Tucker, Zachary Loeliger, Kelsey Padilla-Nash, Hesed Yau, Yu Ying Ried, Thomas Leitman, Susan F. Young, Neal S. Sloand, Elaine M. |
| description | BACKGROUND: Reports of Monosomy 7 in patients receiving granulocyte–colony‐stimulating factor (G‐CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G‐CSF produces Monosomy 7 aneuploidy in healthy donors.
STUDY DESIGN AND METHODS: We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G‐CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G‐CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.
RESULTS: We found no abnormalities in Chromosomes 7 and 8 in G‐CSF–mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G‐CSF– and Dex‐treated donors.
CONCLUSION: G‐CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors. |
| doi_str_mv | 10.1111/j.1537-2995.2011.03313.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3235244</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1020852589</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5743-77ef663b13fa58bb061cefd604231dffc46aec48fcb2b9bc6c8edd33c62c67f63</originalsourceid><addsrcrecordid>eNqNkUuP0zAUhS0EYsrAX0DeILFJ8COx0wVIaDQPxPDQaBBLy3Hs1sWJi-1As54_jkNLGXZ4Y0v3O-de3wMAxKjE-bzalLimvCDLZV0ShHGJKMW03D0Ai2PhIVggVOECY0pOwJMYNwghskT4MTghuGko4WgB7s6N0SpF6A1cBTmMzqsp6UJ554epiMn2o5PJDitopEo-QD_AD37w0fcT5FAOetw6b7sJ2gGutXRpPeW7l8lvvdXJKhiT7qHSzmW6u98FdtkoxKfgkZEu6meH-xR8uTi_Pbsqrj9dvjt7e12omle04FwbxmiLqZF107aIYaVNx1BFKO6MURWTWlWNUS1pl61iqtFdR6liRDFuGD0Fb_a-27Htdaf0kIJ0YhtsL8MkvLTi38pg12LlfwhKaE2qKhu8PBgE_33UMYnexvljeQt-jAIjgpqa1M0yo80eVcHHGLQ5tsFIzBmKjZijEnNUYs5Q_M5Q7LL0-f0xj8I_oWXgxQGQUUln8kKVjX-5mlWc4yZzr_fcT-v09N8DiNubi_mV9cVeb3OCu6Nehm-Cccpr8fXjpXhfEYQadiM-019MBsvR</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1020852589</pqid></control><display><type>article</type><title>Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Olnes, Matthew J. ; Poon, Andrea ; Miranda, Susan J. ; Pfannes, Loretta ; Tucker, Zachary ; Loeliger, Kelsey ; Padilla-Nash, Hesed ; Yau, Yu Ying ; Ried, Thomas ; Leitman, Susan F. ; Young, Neal S. ; Sloand, Elaine M.</creator><creatorcontrib>Olnes, Matthew J. ; Poon, Andrea ; Miranda, Susan J. ; Pfannes, Loretta ; Tucker, Zachary ; Loeliger, Kelsey ; Padilla-Nash, Hesed ; Yau, Yu Ying ; Ried, Thomas ; Leitman, Susan F. ; Young, Neal S. ; Sloand, Elaine M.</creatorcontrib><description>BACKGROUND: Reports of Monosomy 7 in patients receiving granulocyte–colony‐stimulating factor (G‐CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G‐CSF produces Monosomy 7 aneuploidy in healthy donors.
STUDY DESIGN AND METHODS: We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G‐CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G‐CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.
RESULTS: We found no abnormalities in Chromosomes 7 and 8 in G‐CSF–mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G‐CSF– and Dex‐treated donors.
CONCLUSION: G‐CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/j.1537-2995.2011.03313.x</identifier><identifier>PMID: 21883270</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Aneuploidy ; Biological and medical sciences ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Chromosome Deletion ; Chromosomes, Human, Pair 7 - drug effects ; Dexamethasone - administration & dosage ; Genomic Instability - drug effects ; Glucocorticoids - administration & dosage ; Granulocyte Colony-Stimulating Factor - administration & dosage ; Granulocytes - drug effects ; Granulocytes - physiology ; Granulocytes - transplantation ; Hematopoietic Stem Cell Mobilization - adverse effects ; Hematopoietic Stem Cell Mobilization - methods ; Hematopoietic Stem Cell Mobilization - standards ; Hematopoietic Stem Cell Transplantation - methods ; Hematopoietic Stem Cells - drug effects ; Hematopoietic Stem Cells - physiology ; Humans ; Medical sciences ; Tissue Donors ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Transfusion (Philadelphia, Pa.), 2012-03, Vol.52 (3), p.537-541</ispartof><rights>2011 American Association of Blood Banks</rights><rights>2015 INIST-CNRS</rights><rights>2011 American Association of Blood Banks.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5743-77ef663b13fa58bb061cefd604231dffc46aec48fcb2b9bc6c8edd33c62c67f63</citedby><cites>FETCH-LOGICAL-c5743-77ef663b13fa58bb061cefd604231dffc46aec48fcb2b9bc6c8edd33c62c67f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1537-2995.2011.03313.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1537-2995.2011.03313.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25647718$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21883270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olnes, Matthew J.</creatorcontrib><creatorcontrib>Poon, Andrea</creatorcontrib><creatorcontrib>Miranda, Susan J.</creatorcontrib><creatorcontrib>Pfannes, Loretta</creatorcontrib><creatorcontrib>Tucker, Zachary</creatorcontrib><creatorcontrib>Loeliger, Kelsey</creatorcontrib><creatorcontrib>Padilla-Nash, Hesed</creatorcontrib><creatorcontrib>Yau, Yu Ying</creatorcontrib><creatorcontrib>Ried, Thomas</creatorcontrib><creatorcontrib>Leitman, Susan F.</creatorcontrib><creatorcontrib>Young, Neal S.</creatorcontrib><creatorcontrib>Sloand, Elaine M.</creatorcontrib><title>Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>BACKGROUND: Reports of Monosomy 7 in patients receiving granulocyte–colony‐stimulating factor (G‐CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G‐CSF produces Monosomy 7 aneuploidy in healthy donors.
STUDY DESIGN AND METHODS: We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G‐CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G‐CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.
RESULTS: We found no abnormalities in Chromosomes 7 and 8 in G‐CSF–mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G‐CSF– and Dex‐treated donors.
CONCLUSION: G‐CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Aneuploidy</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Chromosome Deletion</subject><subject>Chromosomes, Human, Pair 7 - drug effects</subject><subject>Dexamethasone - administration & dosage</subject><subject>Genomic Instability - drug effects</subject><subject>Glucocorticoids - administration & dosage</subject><subject>Granulocyte Colony-Stimulating Factor - administration & dosage</subject><subject>Granulocytes - drug effects</subject><subject>Granulocytes - physiology</subject><subject>Granulocytes - transplantation</subject><subject>Hematopoietic Stem Cell Mobilization - adverse effects</subject><subject>Hematopoietic Stem Cell Mobilization - methods</subject><subject>Hematopoietic Stem Cell Mobilization - standards</subject><subject>Hematopoietic Stem Cell Transplantation - methods</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Tissue Donors</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuP0zAUhS0EYsrAX0DeILFJ8COx0wVIaDQPxPDQaBBLy3Hs1sWJi-1As54_jkNLGXZ4Y0v3O-de3wMAxKjE-bzalLimvCDLZV0ShHGJKMW03D0Ai2PhIVggVOECY0pOwJMYNwghskT4MTghuGko4WgB7s6N0SpF6A1cBTmMzqsp6UJ554epiMn2o5PJDitopEo-QD_AD37w0fcT5FAOetw6b7sJ2gGutXRpPeW7l8lvvdXJKhiT7qHSzmW6u98FdtkoxKfgkZEu6meH-xR8uTi_Pbsqrj9dvjt7e12omle04FwbxmiLqZF107aIYaVNx1BFKO6MURWTWlWNUS1pl61iqtFdR6liRDFuGD0Fb_a-27Htdaf0kIJ0YhtsL8MkvLTi38pg12LlfwhKaE2qKhu8PBgE_33UMYnexvljeQt-jAIjgpqa1M0yo80eVcHHGLQ5tsFIzBmKjZijEnNUYs5Q_M5Q7LL0-f0xj8I_oWXgxQGQUUln8kKVjX-5mlWc4yZzr_fcT-v09N8DiNubi_mV9cVeb3OCu6Nehm-Cccpr8fXjpXhfEYQadiM-019MBsvR</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Olnes, Matthew J.</creator><creator>Poon, Andrea</creator><creator>Miranda, Susan J.</creator><creator>Pfannes, Loretta</creator><creator>Tucker, Zachary</creator><creator>Loeliger, Kelsey</creator><creator>Padilla-Nash, Hesed</creator><creator>Yau, Yu Ying</creator><creator>Ried, Thomas</creator><creator>Leitman, Susan F.</creator><creator>Young, Neal S.</creator><creator>Sloand, Elaine M.</creator><general>Blackwell Publishing Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>201203</creationdate><title>Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors</title><author>Olnes, Matthew J. ; Poon, Andrea ; Miranda, Susan J. ; Pfannes, Loretta ; Tucker, Zachary ; Loeliger, Kelsey ; Padilla-Nash, Hesed ; Yau, Yu Ying ; Ried, Thomas ; Leitman, Susan F. ; Young, Neal S. ; Sloand, Elaine M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5743-77ef663b13fa58bb061cefd604231dffc46aec48fcb2b9bc6c8edd33c62c67f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Aneuploidy</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Bone marrow, stem cells transplantation. Graft versus host reaction</topic><topic>Chromosome Deletion</topic><topic>Chromosomes, Human, Pair 7 - drug effects</topic><topic>Dexamethasone - administration & dosage</topic><topic>Genomic Instability - drug effects</topic><topic>Glucocorticoids - administration & dosage</topic><topic>Granulocyte Colony-Stimulating Factor - administration & dosage</topic><topic>Granulocytes - drug effects</topic><topic>Granulocytes - physiology</topic><topic>Granulocytes - transplantation</topic><topic>Hematopoietic Stem Cell Mobilization - adverse effects</topic><topic>Hematopoietic Stem Cell Mobilization - methods</topic><topic>Hematopoietic Stem Cell Mobilization - standards</topic><topic>Hematopoietic Stem Cell Transplantation - methods</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Tissue Donors</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olnes, Matthew J.</creatorcontrib><creatorcontrib>Poon, Andrea</creatorcontrib><creatorcontrib>Miranda, Susan J.</creatorcontrib><creatorcontrib>Pfannes, Loretta</creatorcontrib><creatorcontrib>Tucker, Zachary</creatorcontrib><creatorcontrib>Loeliger, Kelsey</creatorcontrib><creatorcontrib>Padilla-Nash, Hesed</creatorcontrib><creatorcontrib>Yau, Yu Ying</creatorcontrib><creatorcontrib>Ried, Thomas</creatorcontrib><creatorcontrib>Leitman, Susan F.</creatorcontrib><creatorcontrib>Young, Neal S.</creatorcontrib><creatorcontrib>Sloand, Elaine M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olnes, Matthew J.</au><au>Poon, Andrea</au><au>Miranda, Susan J.</au><au>Pfannes, Loretta</au><au>Tucker, Zachary</au><au>Loeliger, Kelsey</au><au>Padilla-Nash, Hesed</au><au>Yau, Yu Ying</au><au>Ried, Thomas</au><au>Leitman, Susan F.</au><au>Young, Neal S.</au><au>Sloand, Elaine M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2012-03</date><risdate>2012</risdate><volume>52</volume><issue>3</issue><spage>537</spage><epage>541</epage><pages>537-541</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>BACKGROUND: Reports of Monosomy 7 in patients receiving granulocyte–colony‐stimulating factor (G‐CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G‐CSF produces Monosomy 7 aneuploidy in healthy donors.
STUDY DESIGN AND METHODS: We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G‐CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G‐CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.
RESULTS: We found no abnormalities in Chromosomes 7 and 8 in G‐CSF–mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G‐CSF– and Dex‐treated donors.
CONCLUSION: G‐CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>21883270</pmid><doi>10.1111/j.1537-2995.2011.03313.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-1132 |
| ispartof | Transfusion (Philadelphia, Pa.), 2012-03, Vol.52 (3), p.537-541 |
| issn | 0041-1132 1537-2995 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3235244 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Aneuploidy Biological and medical sciences Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Bone marrow, stem cells transplantation. Graft versus host reaction Chromosome Deletion Chromosomes, Human, Pair 7 - drug effects Dexamethasone - administration & dosage Genomic Instability - drug effects Glucocorticoids - administration & dosage Granulocyte Colony-Stimulating Factor - administration & dosage Granulocytes - drug effects Granulocytes - physiology Granulocytes - transplantation Hematopoietic Stem Cell Mobilization - adverse effects Hematopoietic Stem Cell Mobilization - methods Hematopoietic Stem Cell Mobilization - standards Hematopoietic Stem Cell Transplantation - methods Hematopoietic Stem Cells - drug effects Hematopoietic Stem Cells - physiology Humans Medical sciences Tissue Donors Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T08%3A46%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20granulocyte-colony-stimulating%20factor%20on%20Monosomy%207%20aneuploidy%20in%20healthy%20hematopoietic%20stem%20cell%20and%20granulocyte%20donors&rft.jtitle=Transfusion%20(Philadelphia,%20Pa.)&rft.au=Olnes,%20Matthew%20J.&rft.date=2012-03&rft.volume=52&rft.issue=3&rft.spage=537&rft.epage=541&rft.pages=537-541&rft.issn=0041-1132&rft.eissn=1537-2995&rft.coden=TRANAT&rft_id=info:doi/10.1111/j.1537-2995.2011.03313.x&rft_dat=%3Cproquest_pubme%3E1020852589%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1020852589&rft_id=info:pmid/21883270&rfr_iscdi=true |