Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors

BACKGROUND: Reports of Monosomy 7 in patients receiving granulocyte–colony‐stimulating factor (G‐CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G‐CSF produces Monosomy 7 aneuploidy in healthy donors. STUDY DESIGN AND METHODS: We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G‐CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G‐CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8. RESULTS: We found no abnormalities in Chromosomes 7 and 8 in G‐CSF–mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G‐CSF– and Dex‐treated donors. CONCLUSION: G‐CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.