PINCH-2 expression in cancers involving serosal effusions using quantitative PCR

Y. Yuan, H. P. Dong, D. A. Nymoen, J. M. Nesland, C. Wu and B. Davidson PINCH-2 expression in cancers involving serosal effusions using quantitative PCR Objective: The PINCH-2 gene was previously shown to be overexpressed in malignant mesothelioma compared with ovarian/peritoneal serous carcinoma in...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cytopathology (Oxford) 2011-02, Vol.22 (1), p.22-29
Hauptverfasser:	Yuan, Y, Dong, H.P, Nymoen, D.A, Nesland, J.M, Wu, C, Davidson, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing adenocarcinoma Adenocarcinoma - diagnosis Adenocarcinoma - genetics Adenocarcinoma - pathology Biomarkers, Tumor - analysis Breast Neoplasms - diagnosis Breast Neoplasms - genetics Breast Neoplasms - pathology cytology Diagnosis, Differential DNA Primers DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Exudates and Transudates Female Flow Cytometry Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans LIM Domain Proteins Male malignant mesothelioma Membrane Proteins Mesothelioma - diagnosis Mesothelioma - genetics Mesothelioma - pathology Neoplasms, Cystic, Mucinous, and Serous - diagnosis Neoplasms, Cystic, Mucinous, and Serous - genetics Neoplasms, Cystic, Mucinous, and Serous - pathology Ovarian Neoplasms - diagnosis Ovarian Neoplasms - genetics Ovarian Neoplasms - pathology PINCH-2 Polymerase Chain Reaction quantitative PCR RNA, Messenger - metabolism serous effusions
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Y. Yuan, H. P. Dong, D. A. Nymoen, J. M. Nesland, C. Wu and B. Davidson PINCH-2 expression in cancers involving serosal effusions using quantitative PCR Objective: The PINCH-2 gene was previously shown to be overexpressed in malignant mesothelioma compared with ovarian/peritoneal serous carcinoma in Affymetrix array analysis. The objective of the present study was to validate this finding at the mRNA and protein level. Methods: Effusions (n = 91; 71 ovarian and 10 breast carcinomas, 10 malignant mesotheliomas) were assayed for PINCH-2 mRNA expression using quantitative PCR. PINCH-2 protein expression was analysed in 37 effusions using flow cytometry. Results: Quantitative PCR analysis showed significantly higher PINCH-2 mRNA levels in mesotheliomas compared with carcinomas (P = 0.004). Values of
ISSN:	0956-5507 1365-2303
DOI:	10.1111/j.1365-2303.2010.00757.x