Unique angiogenic and vasculogenic properties of renal cell carcinoma in a xenograft model of bone metastasis are associated with high levels of vegf-a and decreased ang-1 expression
Management of various tumor metastases to bone has dramatically improved, but this is not so for renal cell carcinoma (RCC), which is a difficult surgical problem due to its great vascularity. Furthermore, the unique mechanisms that mediate RCC vasculogenesis in bone remain unknown. To understand th...
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Veröffentlicht in: | Journal of orthopaedic research 2012-02, Vol.30 (2), p.325-333 |
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Sprache: | eng |
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Zusammenfassung: | Management of various tumor metastases to bone has dramatically improved, but this is not so for renal cell carcinoma (RCC), which is a difficult surgical problem due to its great vascularity. Furthermore, the unique mechanisms that mediate RCC vasculogenesis in bone remain unknown. To understand this process we developed a xenograft model that recapitulates highly vascular RCC versus less vascular tumors that metastasize to bone. Human tumor cell lines of RCC (786‐O), prostate cancer (PC3), lung cancer (A549), breast cancer (MDA‐MB231), and melanoma (A375) were transduced with firefly luciferase (Luc), injected into the tibiae of nude mice, and differences in growth, osteolysis, and vascularity were assessed by longitudinal bioluminescent imaging, micro‐CT for measurement of calcified tissues and vascularity and histology. The results showed that while RCC‐Luc has reduced growth and osteolytic potential versus the other tumor lines, it displayed a significant increase in vascular volume (p |
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ISSN: | 0736-0266 1554-527X |
DOI: | 10.1002/jor.21500 |