The nucleotide sequence of a glutamate tRNA from rat liver

A glutamate tRNA from rat liver was purified. By means of post-labeling techniques, its nuccleotide sequence was shown to be: pU-C-C-C-A-C-A-U-m1G-G-U-C-ψ-A-G-C-G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-ψ-U-mem5S2U-U-C-A-C-C-C-A-G-G-C-G-G-C-m5C-m5C-G-G-G-Tm-ψ-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH. The seq...

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Veröffentlicht in:	Nucleic acids research 1982-08, Vol.10 (15), p.4605-4608
Hauptverfasser:	Chan, Jane C., Yang, Julia A., Dunn, Michael J., Agris, Paul F., Wong, Ting-Wa
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Drosophila melanogaster - analysis Liver - analysis Nucleic Acid Conformation Rats Rats, Inbred BUF RNA, Transfer, Amino Acyl - isolation & purification Species Specificity
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Zusammenfassung:	A glutamate tRNA from rat liver was purified. By means of post-labeling techniques, its nuccleotide sequence was shown to be: pU-C-C-C-A-C-A-U-m1G-G-U-C-ψ-A-G-C-G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-ψ-U-mem5S2U-U-C-A-C-C-C-A-G-G-C-G-G-C-m5C-m5C-G-G-G-Tm-ψ-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH. The sequence is remarkably similar to that of tRNA from Drosophila melanogaster. Only 10 out of 75 nucleotides in the two tRNAs are different.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/10.15.4605