Nucleic acid sequence and chromosome assignment of a wheat storage protein gene

A cloned gliadin gene was isolated from a wheat genomic library, and 2.4 kb of its primary sequence determined. The gene, α-1Y, was found by Southern analysis to be located on chromosome 6A, and its derived amino acid sequence identifies it as a member of the A-gliadin subgroup of α-gliadins located on the short arm of that chromosome. α−1Y is apparently functional, and contains consensus TATA and CAAT boxes, and poly-adenylatlon signals. This gliadin gene has no introns, and its noncoding flanking regions contain several short repeats and inverted sequences. The gene is contained in a 6.2 kb EcoRI genomic fragment whose apparent copy number varies in different wheat cultivars.