Lysogenization of ultraviolet-irradiated Escherichia coli with λ: dependence on the recA and recB gene products

The lysogenization of ultraviolet-irradiated Escherichia coli cells by the λ. phage was studied. Genetic analysis indicates that changes in the number of the lysogenized cell during post-UV growth is primarily due to the change in the proteolytic activity of RecA protein. Full proteolytic activity i...

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Veröffentlicht in:	Nucleic acids research 1984-02, Vol.12 (3), p.1563-1572
Hauptverfasser:	Salaj-Smic, E, Serman, D, Vlahovic, M, Petranovic, M, Petranovic, D, Trgovcevic, Z
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Bacteriophage lambda - genetics Bacteriophage lambda - radiation effects Biological and medical sciences Escherichia coli Escherichia coli - genetics Escherichia coli - radiation effects Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases - genetics Fundamental and applied biological sciences. Psychology Genes - radiation effects Genes, Bacterial - radiation effects Genetics Kinetics Lysogeny Microbiology phage lambda Rec A Recombinases - radiation effects Ultraviolet Rays
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Zusammenfassung:	The lysogenization of ultraviolet-irradiated Escherichia coli cells by the λ. phage was studied. Genetic analysis indicates that changes in the number of the lysogenized cell during post-UV growth is primarily due to the change in the proteolytic activity of RecA protein. Full proteolytic activity is achieved only in the presence of the functional recB gene product.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/12.3.1563