Image defocus and altered retinal gene expression in chick: clues to the pathogenesis of ametropia
Because of the retina's role in refractive development, this study was conducted to analyze the retinal transcriptome in chicks wearing a spectacle lens, a well-established means of inducing refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses a...
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Veröffentlicht in: | Investigative ophthalmology & visual science 2011-07, Vol.52 (8), p.5765-5777 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Because of the retina's role in refractive development, this study was conducted to analyze the retinal transcriptome in chicks wearing a spectacle lens, a well-established means of inducing refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses about refractive development.
One-week-old white Leghorn chicks wore a unilateral spectacle lens of +15 or -15 D for 6 hours or 3 days. With total RNA from the retina/(retinal pigment epithelium, RPE), chicken gene microarrays were used to compare gene expression levels between lens-wearing and contralateral control eyes (n = 6 chicks for each condition). Normalized microarray signal intensities were evaluated by analysis of variance, using a false discovery rate of |
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ISSN: | 0146-0404 1552-5783 |
DOI: | 10.1167/iovs.10-6727 |