Image defocus and altered retinal gene expression in chick: clues to the pathogenesis of ametropia

Because of the retina's role in refractive development, this study was conducted to analyze the retinal transcriptome in chicks wearing a spectacle lens, a well-established means of inducing refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses a...

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Veröffentlicht in:Investigative ophthalmology & visual science 2011-07, Vol.52 (8), p.5765-5777
Hauptverfasser: Stone, Richard A, McGlinn, Alice M, Baldwin, Donald A, Tobias, John W, Iuvone, P Michael, Khurana, Tejvir S
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Sprache:eng
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Zusammenfassung:Because of the retina's role in refractive development, this study was conducted to analyze the retinal transcriptome in chicks wearing a spectacle lens, a well-established means of inducing refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses about refractive development. One-week-old white Leghorn chicks wore a unilateral spectacle lens of +15 or -15 D for 6 hours or 3 days. With total RNA from the retina/(retinal pigment epithelium, RPE), chicken gene microarrays were used to compare gene expression levels between lens-wearing and contralateral control eyes (n = 6 chicks for each condition). Normalized microarray signal intensities were evaluated by analysis of variance, using a false discovery rate of
ISSN:0146-0404
1552-5783
DOI:10.1167/iovs.10-6727