Biohybrid Carbon Nanotube/Agarose Fibers for Neural Tissue Engineering

A novel approach for producing carbon nanotube fibers (CNF) composed with the polysaccharide agarose is reported. Current attempts to make CNFs require the use of a polymer or precipitating agent in the coagulating bath that may have negative effects in biomedical applications. It is shown that, by taking advantage of the gelation properties of agarose, one can substitute the bath with distilled water or ethanol and, hence, reduce the complexity associated with alternating the bath components or the use of organic solvents. It is also demonstrated that these CNF can be chemically functionalized to express biological moieties through available free hydroxyl groups in agarose. Agarose CNF are not only conductive and nontoxic; in addition, their functionalization is shown to facilitate cell attachment and response both in vitro and in vivo. Our findings suggest that agarose/CNT hybrid materials are excellent candidates for applications involving neural tissue engineering and biointerfacing with the nervous system. A novel approach for producing carbon nanotube fibers (CNF) composed with the polysaccharide agarose is reported. The TOC image shows representative immunohistochemical images of rat brain slices after insertion of CNT/agarose fiber‐electrodes. Cell types shown are microglia (blue), astrocytes (orange), and neurons (green). A is a control electrode and B is an electrode functionalized with laminin, a neural extracellular matrix protein. Close inspection and quantification of the cell response reveals favorable tissue reaction to the laminin tethered electrode.