Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and s...
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| Veröffentlicht in: | The Journal of infectious diseases 2011-09, Vol.204 (6), p.937-941 |
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| container_title | The Journal of infectious diseases |
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| creator | Kobayashi, Scott D. Malachowa, Natalia Whitney, Adeline R. Braughton, Kevin R. Gardner, Donald J. Long, Dan Wardenburg, Juliane Bubeck Schneewind, Olaf Otto, Michael DeLeo, Frank R. |
| description | Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected. |
| doi_str_mv | 10.1093/infdis/jir441 |
| format | Article |
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The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/jir441</identifier><identifier>PMID: 21849291</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Abscess - microbiology ; Abscess - pathology ; Abscesses ; Animal models ; Animals ; BACTERIA ; Bacterial Load ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - genetics ; Bacterial Toxins - metabolism ; Biological and medical sciences ; Disease models ; Disease Models, Animal ; Exotoxins - genetics ; Exotoxins - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Hemolysin Proteins - genetics ; Hemolysin Proteins - metabolism ; Histocytochemistry ; Infections ; Infectious diseases ; Inoculation ; Leukocidins - genetics ; Leukocidins - metabolism ; Major and Brief Reports ; Medical sciences ; Methicillin-Resistant Staphylococcus aureus - pathogenicity ; Microbiology ; Microscopy ; Neutrophils ; Rabbits ; Skin - microbiology ; Skin - pathology ; Soft Tissue Infections - microbiology ; Soft Tissue Infections - pathology ; Staphylococcal Skin Infections - microbiology ; Staphylococcal Skin Infections - pathology ; Staphylococcus aureus ; Toxins ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Virulence ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>The Journal of infectious diseases, 2011-09, Vol.204 (6), p.937-941</ispartof><rights>Copyright © 2011 Oxford University Press on behalf of the Infectious Diseases Society of America</rights><rights>2015 INIST-CNRS</rights><rights>Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2011. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-c081991adb517b6c285227fabd15ce8cb005631a01b0fdaafe32b88a1c918fc43</citedby><cites>FETCH-LOGICAL-c504t-c081991adb517b6c285227fabd15ce8cb005631a01b0fdaafe32b88a1c918fc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41230664$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41230664$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24575107$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21849291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobayashi, Scott D.</creatorcontrib><creatorcontrib>Malachowa, Natalia</creatorcontrib><creatorcontrib>Whitney, Adeline R.</creatorcontrib><creatorcontrib>Braughton, Kevin R.</creatorcontrib><creatorcontrib>Gardner, Donald J.</creatorcontrib><creatorcontrib>Long, Dan</creatorcontrib><creatorcontrib>Wardenburg, Juliane Bubeck</creatorcontrib><creatorcontrib>Schneewind, Olaf</creatorcontrib><creatorcontrib>Otto, Michael</creatorcontrib><creatorcontrib>DeLeo, Frank R.</creatorcontrib><title>Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.</description><subject>Abscess - microbiology</subject><subject>Abscess - pathology</subject><subject>Abscesses</subject><subject>Animal models</subject><subject>Animals</subject><subject>BACTERIA</subject><subject>Bacterial Load</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Disease models</subject><subject>Disease Models, Animal</subject><subject>Exotoxins - genetics</subject><subject>Exotoxins - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemolysin Proteins - genetics</subject><subject>Hemolysin Proteins - metabolism</subject><subject>Histocytochemistry</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Inoculation</subject><subject>Leukocidins - genetics</subject><subject>Leukocidins - metabolism</subject><subject>Major and Brief Reports</subject><subject>Medical sciences</subject><subject>Methicillin-Resistant Staphylococcus aureus - pathogenicity</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>Neutrophils</subject><subject>Rabbits</subject><subject>Skin - microbiology</subject><subject>Skin - pathology</subject><subject>Soft Tissue Infections - microbiology</subject><subject>Soft Tissue Infections - pathology</subject><subject>Staphylococcal Skin Infections - microbiology</subject><subject>Staphylococcal Skin Infections - pathology</subject><subject>Staphylococcus aureus</subject><subject>Toxins</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>Virulence</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1vEzEUxC0EomnhyBHkC8elfv7YXV-QohRKpSIk0nJdPXttcNjYke1U6n9Poi0RnJ705jcz0hDyBtgHYFpchujHUC43IUsJz8gClOiatgXxnCwY47yBXuszcl7KhjEmRdu9JGcceqm5hgUJq7TdYcYaHhxdRpweSyg0eXq_XgrG6I-Q95OL1tErV13ehoixFhoiRfodjQmVfk2jm46W9e_jO450nXyld6GUvaM30TtbQ4qvyAuPU3Gvn-4Fuf_86W71pbn9dn2zWt42VjFZG8t60BpwNAo601reK847j2YEZV1vDWOqFYAMDPMjoneCm75HsBp6b6W4IB_n3N3ebN1oXawZp2GXwxbz45AwDP8rMfwafqaHQYBqNe8OAc0cYHMqJTt_8gIbjpsP8-bDvPmBf_dv4Yn-O_IBeP8EYLE4-YzRHuwnTqpOATsWv525Takpn3QJXLC2leIPt_iXbw</recordid><startdate>20110915</startdate><enddate>20110915</enddate><creator>Kobayashi, Scott D.</creator><creator>Malachowa, Natalia</creator><creator>Whitney, Adeline R.</creator><creator>Braughton, Kevin R.</creator><creator>Gardner, Donald J.</creator><creator>Long, Dan</creator><creator>Wardenburg, Juliane Bubeck</creator><creator>Schneewind, Olaf</creator><creator>Otto, Michael</creator><creator>DeLeo, Frank R.</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20110915</creationdate><title>Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection</title><author>Kobayashi, Scott D. ; Malachowa, Natalia ; Whitney, Adeline R. ; Braughton, Kevin R. ; Gardner, Donald J. ; Long, Dan ; Wardenburg, Juliane Bubeck ; Schneewind, Olaf ; Otto, Michael ; DeLeo, Frank R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-c081991adb517b6c285227fabd15ce8cb005631a01b0fdaafe32b88a1c918fc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abscess - microbiology</topic><topic>Abscess - pathology</topic><topic>Abscesses</topic><topic>Animal models</topic><topic>Animals</topic><topic>BACTERIA</topic><topic>Bacterial Load</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacterial Toxins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Disease models</topic><topic>Disease Models, Animal</topic><topic>Exotoxins - genetics</topic><topic>Exotoxins - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemolysin Proteins - genetics</topic><topic>Hemolysin Proteins - metabolism</topic><topic>Histocytochemistry</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Inoculation</topic><topic>Leukocidins - genetics</topic><topic>Leukocidins - metabolism</topic><topic>Major and Brief Reports</topic><topic>Medical sciences</topic><topic>Methicillin-Resistant Staphylococcus aureus - pathogenicity</topic><topic>Microbiology</topic><topic>Microscopy</topic><topic>Neutrophils</topic><topic>Rabbits</topic><topic>Skin - microbiology</topic><topic>Skin - pathology</topic><topic>Soft Tissue Infections - microbiology</topic><topic>Soft Tissue Infections - pathology</topic><topic>Staphylococcal Skin Infections - microbiology</topic><topic>Staphylococcal Skin Infections - pathology</topic><topic>Staphylococcus aureus</topic><topic>Toxins</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Virulence</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Scott D.</creatorcontrib><creatorcontrib>Malachowa, Natalia</creatorcontrib><creatorcontrib>Whitney, Adeline R.</creatorcontrib><creatorcontrib>Braughton, Kevin R.</creatorcontrib><creatorcontrib>Gardner, Donald J.</creatorcontrib><creatorcontrib>Long, Dan</creatorcontrib><creatorcontrib>Wardenburg, Juliane Bubeck</creatorcontrib><creatorcontrib>Schneewind, Olaf</creatorcontrib><creatorcontrib>Otto, Michael</creatorcontrib><creatorcontrib>DeLeo, Frank R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Scott D.</au><au>Malachowa, Natalia</au><au>Whitney, Adeline R.</au><au>Braughton, Kevin R.</au><au>Gardner, Donald J.</au><au>Long, Dan</au><au>Wardenburg, Juliane Bubeck</au><au>Schneewind, Olaf</au><au>Otto, Michael</au><au>DeLeo, Frank R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>2011-09-15</date><risdate>2011</risdate><volume>204</volume><issue>6</issue><spage>937</spage><epage>941</epage><pages>937-941</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>21849291</pmid><doi>10.1093/infdis/jir441</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 2011-09, Vol.204 (6), p.937-941 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Abscess - microbiology Abscess - pathology Abscesses Animal models Animals BACTERIA Bacterial Load Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - genetics Bacterial Toxins - metabolism Biological and medical sciences Disease models Disease Models, Animal Exotoxins - genetics Exotoxins - metabolism Female Fundamental and applied biological sciences. Psychology Hemolysin Proteins - genetics Hemolysin Proteins - metabolism Histocytochemistry Infections Infectious diseases Inoculation Leukocidins - genetics Leukocidins - metabolism Major and Brief Reports Medical sciences Methicillin-Resistant Staphylococcus aureus - pathogenicity Microbiology Microscopy Neutrophils Rabbits Skin - microbiology Skin - pathology Soft Tissue Infections - microbiology Soft Tissue Infections - pathology Staphylococcal Skin Infections - microbiology Staphylococcal Skin Infections - pathology Staphylococcus aureus Toxins Trans-Activators - genetics Trans-Activators - metabolism Virulence Virulence Factors - genetics Virulence Factors - metabolism |
| title | Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection |
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