Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and s...

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Veröffentlicht in:	The Journal of infectious diseases 2011-09, Vol.204 (6), p.937-941
Hauptverfasser:	Kobayashi, Scott D., Malachowa, Natalia, Whitney, Adeline R., Braughton, Kevin R., Gardner, Donald J., Long, Dan, Wardenburg, Juliane Bubeck, Schneewind, Olaf, Otto, Michael, DeLeo, Frank R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Abscess - microbiology Abscess - pathology Abscesses Animal models Animals BACTERIA Bacterial Load Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - genetics Bacterial Toxins - metabolism Biological and medical sciences Disease models Disease Models, Animal Exotoxins - genetics Exotoxins - metabolism Female Fundamental and applied biological sciences. Psychology Hemolysin Proteins - genetics Hemolysin Proteins - metabolism Histocytochemistry Infections Infectious diseases Inoculation Leukocidins - genetics Leukocidins - metabolism Major and Brief Reports Medical sciences Methicillin-Resistant Staphylococcus aureus - pathogenicity Microbiology Microscopy Neutrophils Rabbits Skin - microbiology Skin - pathology Soft Tissue Infections - microbiology Soft Tissue Infections - pathology Staphylococcal Skin Infections - microbiology Staphylococcal Skin Infections - pathology Staphylococcus aureus Toxins Trans-Activators - genetics Trans-Activators - metabolism Virulence Virulence Factors - genetics Virulence Factors - metabolism
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Beschreibung
Zusammenfassung:	Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.
ISSN:	0022-1899 1537-6613
DOI:	10.1093/infdis/jir441