The aryl hydrocarbon receptor interacts with ATP5α1, a subunit of the ATP synthase complex, and modulates mitochondrial function
Dioxins, including 2,3,7,8 tetrachlorodibenzo- p-dioxin (TCDD), produce a wide range of toxic effects in mammals. Most, if not all, of these toxic effects are regulated by the aryl hydrocarbon receptor (AHR). The AHR is a ligand activated transcription factor that has been shown to interact with num...
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Veröffentlicht in: | Toxicology and applied pharmacology 2011-08, Vol.254 (3), p.299-310 |
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Sprache: | eng |
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Zusammenfassung: | Dioxins, including 2,3,7,8 tetrachlorodibenzo-
p-dioxin (TCDD), produce a wide range of toxic effects in mammals. Most, if not all, of these toxic effects are regulated by the aryl hydrocarbon receptor (AHR). The AHR is a ligand activated transcription factor that has been shown to interact with numerous proteins capable of influencing the receptor's function. The ability of secondary proteins to alter AHR-mediated transcriptional events, a necessary step for toxicity, led us to determine whether additional interacting proteins could be identified. To this end, we have employed tandem affinity purification (TAP) of the AHR in Hepa1c1c7 cells. TAP of the AHR, followed by mass spectrometry (MS) identified ATP5α1, a subunit of the ATP synthase complex, as a strong AHR interactor in the absence of ligand. The interaction was lost upon exposure to TCDD. The association was confirmed by co-immunoprecipitation in multiple cell lines. In addition, cell fractionation experiments showed that a fraction of the AHR is found in the mitochondria. To ascribe a potential functional role to the AHR:ATP5α1 interaction, TCDD was shown to induce a hyperpolarization of the mitochondrial membrane in an AHR-dependent and transcription-independent manner. These results suggest that a fraction of the total cellular AHR pool is localized to the mitochondria and contributes to the organelle's homeostasis.
► The AHR interacts with the mitochondrial protein, ATP5α1. ► Cell fractionation experiments show that the AHR can be found in the mitochondria. ► TCDD-exposure induces a hyperpolarization of the mitochondrial inner membrane. ► The hyperpolarization is AHR-dependent. ► The hyperpolarization occurs without altering ATP levels within the cell. |
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ISSN: | 0041-008X 1096-0333 |
DOI: | 10.1016/j.taap.2011.05.004 |