Molecular Detection and Identification of Rickettsia Species in Ixodes pacificus in California

We amplified 16S rRNA, gltA , and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus . While phylotype G021 has high homology to Ixodes scapularis endosymbiotic...

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Veröffentlicht in:	Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2011-07, Vol.11 (7), p.957-961
Hauptverfasser:	Phan, Jimmy Ninh, Lu, Casey Roy, Bender, William Garrett, Smoak, Robert Marion, Zhong, Jianmin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Outer Membrane Proteins - genetics California Databases, Nucleic Acid DNA sequencing Gene expression Genetic aspects Identification and classification Ixodes - microbiology Ixodes pacificus Ixodes scapularis Methods Nucleotide sequencing Physiological aspects Polymerase Chain Reaction Rickettsia Rickettsia - classification Rickettsia - genetics Rickettsia - isolation & purification RNA RNA, Ribosomal, 16S Sequence Analysis Ticks
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Beschreibung
Zusammenfassung:	We amplified 16S rRNA, gltA , and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus . While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia , phylotype G022 is a deeply branched novel spotted fever group Rickettsia .
ISSN:	1530-3667 1557-7759
DOI:	10.1089/vbz.2010.0077