high-capacity column for affinity purification of sequence-specific DNA-binding proteins

We report an improved method for synthesis of a DNA affinity column, involving site-specific linkage of a monomeric or concatenated oligonucleotide to an activated chromatographic support. In the present method, efficient and site-specific coupling is accomplished between DNA bearing a reactive alkylamine-tethered nucleoside and a carboxyl-activated support; attachment of the tether to a DNA base (6-8) and co-synthetic 5'-phosphorylation allow for facile generation of concatenated DNA.