Genetic and Molecular Basis for Sunscreen Biosynthesis in Cyanobacteria

Ultraviolet UV-A and UV-B radiation is harmful to living systems, causing damage to biological macromolecules. An important strategy for dealing with UV exposure is the biosynthesis of small-molecule sunscreens. Among such metabolites, the mycosporine and mycosporine-like amino acids (MAAs) are rema...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2010-09, Vol.329 (5999), p.1653-1656
Hauptverfasser: Balskus, Emily P, Walsh, Christopher T
Format: Artikel
Sprache:eng
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Zusammenfassung:Ultraviolet UV-A and UV-B radiation is harmful to living systems, causing damage to biological macromolecules. An important strategy for dealing with UV exposure is the biosynthesis of small-molecule sunscreens. Among such metabolites, the mycosporine and mycosporine-like amino acids (MAAs) are remarkable for their wide phylogenetic distribution and their unique chemical structures. Here, we report the identification of a MAA biosynthetic gene cluster in a cyanobacterium and the discovery of analogous pathways in other sequenced organisms. We have expressed the cluster in a heterologous bacterial host and characterized all four biosynthetic enzymes in vitro. In addition to clarifying the origin of the MAAs, these efforts have revealed two unprecedented enzymatic strategies for imine formation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1193637