Nonisotopic SSCP and competitive PCR for DNA quantification: p53 in breast cancer cells

We describe here the quantification of the p53 gene and alleles in genomic DNA extracted from MDA cells and human breast cancer biopsies respectively, using a rapid nonisotopic SSCP method.

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Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 1992-01, Vol.20 (1), p.145-145
Hauptverfasser:	Yap, E P, McGee, J O
Format:	Artikel
Sprache:	eng
Schlagworte:	breast Breast Neoplasms - genetics carcinoma DNA, Single-Stranded - genetics Genes, p53 - genetics genomes Humans Nucleic Acid Conformation Polymerase Chain Reaction Polymorphism, Genetic - genetics Tumor Cells, Cultured
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	We describe here the quantification of the p53 gene and alleles in genomic DNA extracted from MDA cells and human breast cancer biopsies respectively, using a rapid nonisotopic SSCP method.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/20.1.145