PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning

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Veröffentlicht in:	Nucleic acids research 1992-01, Vol.20 (1), p.144-144
Hauptverfasser:	Lohff, C.J, Cease, K.B
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology blunt cloning cloning Cloning, Molecular DNA - metabolism DNA-directed DNA polymerase DNA-Directed DNA Polymerase - metabolism enzyme activity Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics In vitro gene amplification. Pcr technique Methods. Procedures. Technologies nucleases polymerase chain reaction Polymerase Chain Reaction - methods Polynucleotide 5'-Hydroxyl-Kinase - metabolism Taq Polymerase
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