Cloning and analysis of the L17 ribosomal protein cDNA from Podocoryne carnea (Coelenterata, Hydrozoa)

The authors report the characterization of a cDNA encoding ribosomal protein L17 from Podocoryne carnea , which was obtained from a differential screening of a cDNA library for genes involved in transdifferentiation of striated to smooth muscle cells of Podocoryne . From the isolation procedure it is suggested that the mRNA for ribosomal protein L17 is present in elevated levels in isolated striated muscle tissues. The cDNA insert has a length of 577 nucleotides. It consists of 10 nucleotides of 5' noncoding sequence, an open reading frame comprising 552 nucleotides and a 3' untranslated region of 33 nucleotides including an 18 nucleotide long poly (A) stretch. The amino acid sequence deduced from the open reading frame encodes a protein of 183 amino acid residues.