A simple and rapid method for the preparation of gram-negative bacterial genomic DNA
Two major protocols for the preparation of bacterial genomic DNA have been commonly used. These methods provided powerful means of preparing genomic DNA; however, both of them are rather complex and time-consuming. Presented here is a simple and rapid method for extraction of bacterial genomic DNA....
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| Veröffentlicht in: | Nucleic acids research 1993-05, Vol.21 (9), p.2260-2260 |
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| container_title | Nucleic acids research |
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| creator | Chen, Wen-ping Kuo, Tsong-teh |
| description | Two major protocols for the preparation of bacterial genomic DNA have been commonly used. These methods provided powerful means of preparing genomic DNA; however, both of them are rather complex and time-consuming. Presented here is a simple and rapid method for extraction of bacterial genomic DNA. This method is effective in producing digestible genomic DNA from a variety of gram-negative bacteria, including those of the genera Xanthomonas, Pseudomonase, Agrobacterium , and Rhizobium . All of them normally produce copious amounts of polysaccharides, the most common problem affecting DNA purity, which can inhibit the activity of many molecular biological enzymes. The method also has several advantages of not requiring any enzymes for expensive cell lysis materials. It requires only one chloroform extraction, and the entire process can be accomplished within one hour. |
| doi_str_mv | 10.1093/nar/21.9.2260 |
| format | Article |
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These methods provided powerful means of preparing genomic DNA; however, both of them are rather complex and time-consuming. Presented here is a simple and rapid method for extraction of bacterial genomic DNA. This method is effective in producing digestible genomic DNA from a variety of gram-negative bacteria, including those of the genera Xanthomonas, Pseudomonase, Agrobacterium , and Rhizobium . All of them normally produce copious amounts of polysaccharides, the most common problem affecting DNA purity, which can inhibit the activity of many molecular biological enzymes. The method also has several advantages of not requiring any enzymes for expensive cell lysis materials. It requires only one chloroform extraction, and the entire process can be accomplished within one hour.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/21.9.2260</identifier><identifier>PMID: 8502576</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Agrobacterium ; Bacteria ; Biological and medical sciences ; Diverse techniques ; DNA, Bacterial - isolation & purification ; Fundamental and applied biological sciences. 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These methods provided powerful means of preparing genomic DNA; however, both of them are rather complex and time-consuming. Presented here is a simple and rapid method for extraction of bacterial genomic DNA. This method is effective in producing digestible genomic DNA from a variety of gram-negative bacteria, including those of the genera Xanthomonas, Pseudomonase, Agrobacterium , and Rhizobium . All of them normally produce copious amounts of polysaccharides, the most common problem affecting DNA purity, which can inhibit the activity of many molecular biological enzymes. The method also has several advantages of not requiring any enzymes for expensive cell lysis materials. It requires only one chloroform extraction, and the entire process can be accomplished within one hour.</description><subject>Agrobacterium</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Diverse techniques</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Techniques</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Molecular and cellular biology</subject><subject>Rhizobium</subject><subject>Xanthomonas</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2P0zAQxS0EWsrCkSOSD4hbuh47tuMDh2r5WERVBFokxMWaOE5rSOKsna7gvyfVVgVOexpp3m9m3ugR8hzYEpgRFwOmCw5Ls-RcsQdkAULxojSKPyQLJpgsgJXVY_Ik5x-MQQmyPCNnlWRcarUg1yuaQz92nuLQ0IRjaGjvp11saBsTnXaejsmPmHAKcaCxpduEfTH47dy49bRGN_kUsKNbP8Q-OPpms3pKHrXYZf_sWM_J13dvry-vivWn9x8uV-vCzbenAlXFAYRphMJSae2AOXAVk064WvJWSQQBpmmVa8GJmtey1ajrCl2jOWvEOXl9t3fc171vnB-mhJ0dU-gx_bYRg_1fGcLObuOtFcxIJub5V8f5FG_2Pk-2D9n5rsPBx322WmqhQZl7QTDalEbD_aBSwCotZ7C4A12KOSffnlwDs4dc7Zyr5WCNPeQ68y_-ffVEH4Oc9ZdHHbPDrk04uJBPWKkPFtXfsyFP_tdJxvTTqvlXaa--fbebL-vPm48KrBJ_AEkwus4</recordid><startdate>19930511</startdate><enddate>19930511</enddate><creator>Chen, Wen-ping</creator><creator>Kuo, Tsong-teh</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7T7</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930511</creationdate><title>A simple and rapid method for the preparation of gram-negative bacterial genomic DNA</title><author>Chen, Wen-ping ; Kuo, Tsong-teh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-a6821139d36a4677c10c1c805c3cb52f65a1319df6cf1c3b2b5f7a7b8acd720d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Agrobacterium</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Diverse techniques</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Techniques</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Molecular and cellular biology</topic><topic>Rhizobium</topic><topic>Xanthomonas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Wen-ping</creatorcontrib><creatorcontrib>Kuo, Tsong-teh</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Wen-ping</au><au>Kuo, Tsong-teh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple and rapid method for the preparation of gram-negative bacterial genomic DNA</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1993-05-11</date><risdate>1993</risdate><volume>21</volume><issue>9</issue><spage>2260</spage><epage>2260</epage><pages>2260-2260</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Two major protocols for the preparation of bacterial genomic DNA have been commonly used. These methods provided powerful means of preparing genomic DNA; however, both of them are rather complex and time-consuming. Presented here is a simple and rapid method for extraction of bacterial genomic DNA. This method is effective in producing digestible genomic DNA from a variety of gram-negative bacteria, including those of the genera Xanthomonas, Pseudomonase, Agrobacterium , and Rhizobium . All of them normally produce copious amounts of polysaccharides, the most common problem affecting DNA purity, which can inhibit the activity of many molecular biological enzymes. The method also has several advantages of not requiring any enzymes for expensive cell lysis materials. It requires only one chloroform extraction, and the entire process can be accomplished within one hour.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>8502576</pmid><doi>10.1093/nar/21.9.2260</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1993-05, Vol.21 (9), p.2260-2260 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_309503 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Agrobacterium Bacteria Biological and medical sciences Diverse techniques DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Genetic Techniques Gram-Negative Bacteria - genetics Molecular and cellular biology Rhizobium Xanthomonas |
| title | A simple and rapid method for the preparation of gram-negative bacterial genomic DNA |
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