A simple and rapid method for the preparation of gram-negative bacterial genomic DNA

Two major protocols for the preparation of bacterial genomic DNA have been commonly used. These methods provided powerful means of preparing genomic DNA; however, both of them are rather complex and time-consuming. Presented here is a simple and rapid method for extraction of bacterial genomic DNA. This method is effective in producing digestible genomic DNA from a variety of gram-negative bacteria, including those of the genera Xanthomonas, Pseudomonase, Agrobacterium , and Rhizobium . All of them normally produce copious amounts of polysaccharides, the most common problem affecting DNA purity, which can inhibit the activity of many molecular biological enzymes. The method also has several advantages of not requiring any enzymes for expensive cell lysis materials. It requires only one chloroform extraction, and the entire process can be accomplished within one hour.