Deprotection of methylphosphonate oligonucleotides using a novel one-pot procedure

Deprotection of methylphosphonate oligonucleotides with ethylenediamine was evaluated in a model system. Methylphosphonate sequences of the form 5′-TTTNNTTT. where N was either N4-bz-dC, N4-lbu-dC, N2-lbu-O6-DPC-dG, N2-ibu-dG, N6-bz-dA, or T, were used to determine the extent of modifications that occur during deprotection. Up to 15% of N4-bz-dC was found to transaminate at the C4 position when treated with ethylenediamine. A similar displacement reaction with ethylenediamine was observed at the O6 position of N2ibu-O6-DPC-dG, and to a much lesser extent of N2-ibu-dG. Side reactions were not observed when oligonucleotides containing N4-lbu-dC, N6-bz-dA, or T were treated with ethylenediamine. A novel method of deprotecting methylphosphonate oligonucleotides was developed from these studies. The method incorporates a brief treatment with dilute ammonia for 30 minutes followed by addition of ethylenediamine for 6 hours at room temperature to complete deprotection in a one-pot format. The solution Is then diluted and neutralized to stop the reaction and prepare the crude product for chromatographlc purification. This method was used to successfully deprotect a series of oligonucleotides at the 1, 100, and 150 μmole scales. These deprotection results were compared to a commonly used two-step method and found to be superior In yield of product by as much as 250%.