Anti-contamination device for cryogenic soft X-ray diffraction microscopy

Cryogenic microscopy allows one to view frozen hydrated biological and soft matter specimens with good structural preservation and a high degree of stability against radiation damage. We describe a liquid nitrogen-cooled anti-contamination device for cryogenic X-ray diffraction microscopy. The anti-contaminator greatly reduces the buildup of ice layers on the specimen due to condensation of residual water vapor in the experimental vacuum chamber. We show by coherent X-ray diffraction measurements that this leads to fivefold reduction of background scattering, which is important for far-field X-ray diffraction microscopy of biological specimens. ► Anti-contamination device for cryogenic X-ray diffraction microscope. ► Reduce background scattering by five folds. ► Enable three-dimensional data acquisition from biological samples at cryogenic temperature.