A method for preparation of fecal DNA suitable for PCR

A method for preparation of fecal DNA suitable for PCR

The analysis of nuclear DNA extracted from fecal specimens is an important tool in different areas of molecular genetic research reaching from cancer diagnostics to population genetic studies. Here we describe a short and simple method for the purification of chromosomal DNA that is ready to be used...

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Veröffentlicht in:Nucleic acids research 1995-09, Vol.23 (18), p.3800-3801
Hauptverfasser: Deuter, Ralner, Pietsch, Stephan, Hertel, Sven, Müller, Oliver
Format: Artikel
Sprache:eng
Schlagworte:
DNA - genetics
DNA - isolation & purification
Feces - chemistry
Flour
Humans
Polymerase Chain Reaction - methods
Solanum tuberosum
Starch
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container_end_page 3801
container_issue 18
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container_title Nucleic acids research
container_volume 23
creator Deuter, Ralner
Pietsch, Stephan
Hertel, Sven
Müller, Oliver
description The analysis of nuclear DNA extracted from fecal specimens is an important tool in different areas of molecular genetic research reaching from cancer diagnostics to population genetic studies. Here we describe a short and simple method for the purification of chromosomal DNA that is ready to be used in PCR. Without further subcloning the resulting PCR products can be used directly for heteroduplex analysis, sequencing or other DNA analysing methods.
doi_str_mv 10.1093/nar/23.18.3800
format Article
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source MEDLINE; Oxford University Press Archive; PubMed Central
subjects DNA - genetics
DNA - isolation & purification
Feces - chemistry
Flour
Humans
Polymerase Chain Reaction - methods
Solanum tuberosum
Starch
title A method for preparation of fecal DNA suitable for PCR
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