A method for preparation of fecal DNA suitable for PCR

The analysis of nuclear DNA extracted from fecal specimens is an important tool in different areas of molecular genetic research reaching from cancer diagnostics to population genetic studies. Here we describe a short and simple method for the purification of chromosomal DNA that is ready to be used...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 1995-09, Vol.23 (18), p.3800-3801
Hauptverfasser:	Deuter, Ralner, Pietsch, Stephan, Hertel, Sven, Müller, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA - genetics DNA - isolation & purification Feces - chemistry Flour Humans Polymerase Chain Reaction - methods Solanum tuberosum Starch
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The analysis of nuclear DNA extracted from fecal specimens is an important tool in different areas of molecular genetic research reaching from cancer diagnostics to population genetic studies. Here we describe a short and simple method for the purification of chromosomal DNA that is ready to be used in PCR. Without further subcloning the resulting PCR products can be used directly for heteroduplex analysis, sequencing or other DNA analysing methods.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/23.18.3800