The cDNA and deduced amino acid sequence of profilin from Saccharomyces cerevisiae
A yeast cDNA library in the vector pMAC561 was screened using a nick-translated fragment carrying coding sequences from the yeast profilin gene as a probe. Four clones were obtained, the longest of which was sequenced. Comparison of presence of an intron of 209 bases in the fifth triplet of the codi...
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| Veröffentlicht in: | Nucleic acids research 1987-11, Vol.15 (21), p.9078-9078 |
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| container_issue | 21 |
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| container_title | Nucleic acids research |
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| creator | OECHSNER, U MAGDOLEN, V BANDLOW, W |
| description | A yeast cDNA library in the vector pMAC561 was screened using a nick-translated fragment carrying coding sequences from the yeast profilin gene as a probe. Four clones were obtained, the longest of which was sequenced. Comparison of presence of an intron of 209 bases in the fifth triplet of the coding sequence. Pronounced domain homology with profilin, an G-actin binding protein sequenced from Acanthamoeba and beef identified the nature of the encoded protein. The cDNA, although containing the entire coding sequence and the 3'- non-translated trailer, is apparently incomplete and lacks a few bases at its 5' end as its starts with a G, not encoded by the genomic DNA. |
| doi_str_mv | 10.1093/nar/15.21.9078 |
| format | Article |
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Four clones were obtained, the longest of which was sequenced. Comparison of presence of an intron of 209 bases in the fifth triplet of the coding sequence. Pronounced domain homology with profilin, an G-actin binding protein sequenced from Acanthamoeba and beef identified the nature of the encoded protein. The cDNA, although containing the entire coding sequence and the 3'- non-translated trailer, is apparently incomplete and lacks a few bases at its 5' end as its starts with a G, not encoded by the genomic DNA.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA</subject><subject>Contractile Proteins</subject><subject>DNA - genetics</subject><subject>DNA, Recombinant</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Genes. Genome</subject><subject>Microfilament Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>profilin</subject><subject>Profilins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctrGzEQxkVpSd20194KOpTe1tHouXvoISR9QUggTc9Cnh3VKrtaV7ID-e-zJsa0p5xm4Pt98-Bj7D2IJYhOneVQzsAsJSw74doXbAHKykZ3Vr5kC6GEaUDo9jV7U-sfIUCD0SfsRClw0pkFu71bE8fL63Mecs976ndIPQ9jyhMPmHpe6e-OMhKfIt-UKaYhZR7LNPKfAXEd5u4BqXKkQveppkBv2asYhkrvDvWU_fr65e7ie3N18-3HxflVg1q5beMACYWRBDZKR6tIFkyUsmtV15mVVUZ1waootbSdslH3FlCKHkWMLUShTtnnp7mb3WqkHilvSxj8pqQxlAc_heT_V3Ja-9_TvVfCzjNn_6eDv0zzj3Xrx1SRhiFkmnbVO9dqB_Pu50AphFVS6WdB0K0Bp_enL59ALFOtheLxahB-H6ufY_VgvAS_j3U2fPj31yN-yHHWPx70UDEMsYSMqR4xZ0SrlVWPk3mqww</recordid><startdate>19871111</startdate><enddate>19871111</enddate><creator>OECHSNER, U</creator><creator>MAGDOLEN, V</creator><creator>BANDLOW, W</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19871111</creationdate><title>The cDNA and deduced amino acid sequence of profilin from Saccharomyces cerevisiae</title><author>OECHSNER, U ; MAGDOLEN, V ; BANDLOW, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-71cec052e16f27ebfe615f22983995b63539a63f2426936f4d61c20dc0ff81f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA</topic><topic>Contractile Proteins</topic><topic>DNA - genetics</topic><topic>DNA, Recombinant</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - genetics</topic><topic>Genes. Genome</topic><topic>Microfilament Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>profilin</topic><topic>Profilins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OECHSNER, U</creatorcontrib><creatorcontrib>MAGDOLEN, V</creatorcontrib><creatorcontrib>BANDLOW, W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OECHSNER, U</au><au>MAGDOLEN, V</au><au>BANDLOW, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cDNA and deduced amino acid sequence of profilin from Saccharomyces cerevisiae</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1987-11-11</date><risdate>1987</risdate><volume>15</volume><issue>21</issue><spage>9078</spage><epage>9078</epage><pages>9078-9078</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>A yeast cDNA library in the vector pMAC561 was screened using a nick-translated fragment carrying coding sequences from the yeast profilin gene as a probe. Four clones were obtained, the longest of which was sequenced. Comparison of presence of an intron of 209 bases in the fifth triplet of the coding sequence. Pronounced domain homology with profilin, an G-actin binding protein sequenced from Acanthamoeba and beef identified the nature of the encoded protein. The cDNA, although containing the entire coding sequence and the 3'- non-translated trailer, is apparently incomplete and lacks a few bases at its 5' end as its starts with a G, not encoded by the genomic DNA.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3317275</pmid><doi>10.1093/nar/15.21.9078</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Nucleic acids research, 1987-11, Vol.15 (21), p.9078-9078 |
| issn | 0305-1048 1362-4962 |
| language | eng |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences cDNA Contractile Proteins DNA - genetics DNA, Recombinant Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Genes. Genome Microfilament Proteins - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequence profilin Profilins Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics |
| title | The cDNA and deduced amino acid sequence of profilin from Saccharomyces cerevisiae |
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