Glutamine depletion induces murine neonatal melena with increased apoptosis of the intestinal epithelium

AIM：To investigate the possible biological outcome and effect of glutamine depletion in neonatal mice and rodent intestinal epithelial cells.METHODS：We developed three kinds of artificial milk with different amounts of glutamine;Complete amino acid milk （CAM）,which is based on maternal mouse milk,glutamine-depleted milk （GDM）,and glutaminerich milk （GRM）.GRM contains three-fold more glutamine than CAM.Eighty-seven newborn mice were divided into three groups and were fed with either of CAM,GDM,or GRM via a recently improved nipple-bottle system for seven days.After the feeding period,the mice were subjected to macroscopic and microscopic observations by immunohistochemistry for 5-bromo-2＇deoxyuridine （BrdU） and Ki-67 as markers of cell proliferation,and for cleaved-caspase-3 as a marker of apoptosis.Moreover,IEC6 rat intestinal epithelial cells were cultured in different concentrations of glutamine and were subject to a 4-[3-（4-iodophenyl）-2-（4-nitrophenyl）2H-5-tetrazolio]-1,3-benzene disulfonate cell proliferation assay,flow cytometry,and western blotting to examine the biological effect of glutamine on cell growth and apoptosis.RESULTS：During the feeding period,we found colonic hemorrhage in six of 28 GDM-fed mice （21.4%）,but not in the GRM-fed mice,with no differences in body weight gain between each group.Microscopic examination showed destruction of microvilli and the disappearance of glycocalyx of the intestinal wall in the colon epithelial tissues taken from GDM-fed mice.Intake of GDM reduced BrdU incorporation （the average percentage of BrdU-positive staining;GRM：13.8%,CAM：10.7%,GDM：1.14%,GRM vs GDM：P 0.001,CAM vs GDM：P 0.001） and Ki-67 labeling index （the average percentage of Ki67-positive staining;GRM：24.5%,CAM：22.4% GDM：19.4%,GRM vs GDM：P=0.001,CAM vs GDM：P =0.049）,suggesting that glutamine depletion inhibited cell proliferation of intestinal epithelial cells.Glutamine deprivation further caused the deformation of the nuclear membrane and the plasma membrane,accompanied by chromatin degeneration and an absence of fat droplets from the colonic epithelia,indicating that the cells underwent apoptosis.Moreover,immunohistochemical analysis revealed the appearance of cleaved caspase-3 in colonic epithelial cells of GDM-fed mice.Finally,when IEC6 rat intestinal epithelial cells were cultured without glutamine,cell proliferation was significantly suppressed after 24 h （relative cell growth;4 mmol/L：100.0% ± 36.1%,0 mmol/L：25.3% ± 25.0%,P 0.05）,with seve