Non-alcoholic fatty liver disease proteomics

Purpose: Non‐alcoholic fatty liver disease (NAFLD) is an important cause of chronic liver injury that has gained concern in clinical hepatology. The principal aim of this study was to find differences in protein expression between patients with NAFLD and healthy controls. Experimental design: Changes in protein expression of liver samples from each of the three groups of subjects, controls, non‐alcoholic steatosis, and non‐alcoholic steatohepatitis (NASH), were analyzed by DIGE combined with MALDI TOF/TOF analysis, a proteomic approach that allows to compare hundreds of proteins simultaneously. Results: Forty‐three proteins exhibiting significant changes (ratio ≥1.5, p