Azido-BODIPY Acid Reveals Quantitative Staudinger-Bertozzi Ligation in Two-Step Activity-Based Proteasome Profiling

Flexibility in protein profiling: Employing the new bifunctional fluorophore azido‐BODIPY acid we have synthesized a compatible set of one‐step and two‐step proteasome probes and demonstrate that with these the efficiency of the Staudinger–Bertozzi ligation in the two‐step activity‐based protein pro...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2008-07, Vol.9 (11), p.1735-1738
Hauptverfasser:	Verdoes, Martijn, Florea, Bogdan I, Hillaert, Ulrik, Willems, Lianne I, van der Linden, Wouter A, Sae-Heng, Myra, Filippov, Dmitri V, Kisselev, Alexei F, van der Marel, Gijsbert A, Overkleeft, Herman S
Format:	Artikel
Sprache:	eng
Schlagworte:	activity-based protein profiling Boron Compounds - chemistry Boron Compounds - metabolism Catalysis Cross-Linking Reagents - metabolism Fluorescence fluorescent probes inhibitors Proteasome Endopeptidase Complex - metabolism proteomics Staudinger ligation Substrate Specificity
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Beschreibung
Zusammenfassung:	Flexibility in protein profiling: Employing the new bifunctional fluorophore azido‐BODIPY acid we have synthesized a compatible set of one‐step and two‐step proteasome probes and demonstrate that with these the efficiency of the Staudinger–Bertozzi ligation in the two‐step activity‐based protein profiling of the proteasome catalytic activities is easily established.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200800231