Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture

Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of beta-Bodipy-FL-C(5)-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This...

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Veröffentlicht in:Journal of biological physics 2006-01, Vol.32 (1), p.SN1-SN4
Hauptverfasser: Wenger, Jérôme, Rigneault, Hervé, Dintinger, José, Marguet, Didier, Lenne, Pierre-François
Format: Artikel
Sprache:eng
Schlagworte:
Biophysics
Cellular Biology
Fluorescence
Life Sciences
Lipids
Membranes
Molecular biology
Optics
Physics
Short Note
Subcellular Processes
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Beschreibung
Zusammenfassung:We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of beta-Bodipy-FL-C(5)-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.
ISSN:0092-0606
1573-0689
DOI:10.1007/s10867-006-2909-x