miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1

Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor e...

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Veröffentlicht in:Genes & development 2011-01, Vol.25 (2), p.119-124
Hauptverfasser: Zhang, Lingbo, Flygare, Johan, Wong, Piu, Lim, Bing, Lodish, Harvey F
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Sprache:eng
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Zusammenfassung:Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, down-regulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1998711