A Major Fraction of Glycosphingolipids in Model and Cellular Cholesterol-containing Membranes Is Undetectable by Their Binding Proteins

A Major Fraction of Glycosphingolipids in Model and Cellular Cholesterol-containing Membranes Is Undetectable by Their Binding Proteins

Glycosphingolipids (GSLs) accumulate in cholesterol-enriched cell membrane domains and provide receptors for protein ligands. Lipid-based “aglycone” interactions can influence GSL carbohydrate epitope presentation. To evaluate this relationship, Verotoxin binding its receptor GSL, globotriaosyl cera...

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Veröffentlicht in:The Journal of biological chemistry 2010-11, Vol.285 (46), p.36049-36059
Hauptverfasser: Mahfoud, Radhia, Manis, Adam, Binnington, Beth, Ackerley, Cameron, Lingwood, Clifford A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Membrane - chemistry
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Chlorocebus aethiops
Cholera
Cholera Toxin - metabolism
Cholesterol - metabolism
Cryoelectron Microscopy
G(M1) Ganglioside - analogs & derivatives
G(M1) Ganglioside - metabolism
Glycobiology and Extracellular Matrices
Glycolipids
Glycosphingolipids - metabolism
HIV Envelope Protein gp120 - metabolism
HIVgp120
Humans
Immunoblotting
Lipid Raft
Membrane Bilayer
Microscopy, Fluorescence
Microscopy, Immunoelectron
Protein Binding
Receptors
Shiga Toxins - metabolism
Toxins
Trihexosylceramides - metabolism
Ultracentrafugation
Vero Cells
Verotoxin
Xenobiotics
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Zusammenfassung:Glycosphingolipids (GSLs) accumulate in cholesterol-enriched cell membrane domains and provide receptors for protein ligands. Lipid-based “aglycone” interactions can influence GSL carbohydrate epitope presentation. To evaluate this relationship, Verotoxin binding its receptor GSL, globotriaosyl ceramide (Gb3), was analyzed in simple GSL/cholesterol, detergent-resistant membrane vesicles by equilibrium density gradient centrifugation. Vesicles separated into two Gb3/cholesterol-containing populations. The lighter, minor fraction (
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M110.110189