Angiotensin II and NADPH Oxidase Increase ADMA in Vascular Smooth Muscle Cells

Asymmetrical dimethylarginine inhibits nitric oxide synthase, cationic amino acid transport, and endothelial function. Patients with cardiovascular risk factors often have endothelial dysfunction associated with increased plasma asymmetrical dimethylarginine and markers of reactive oxygen species. W...

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Veröffentlicht in:	Hypertension (Dallas, Tex. 1979) Tex. 1979), 2010-09, Vol.56 (3), p.498-504
Hauptverfasser:	Luo, Zaiming, Teerlink, Tom, Griendling, Kathy, Aslam, Shakil, Welch, William J, Wilcox, Christopher S
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of Variance Angiotensin II - metabolism Angiotensin II - pharmacology Animals Arginine - analogs & derivatives Arginine - genetics Arginine - metabolism Arterial hypertension. Arterial hypotension Benzimidazoles - pharmacology Biological and medical sciences Blood and lymphatic vessels Blood vessels and receptors Blotting, Western Cardiology. Vascular system Cells, Cultured Fundamental and applied biological sciences. Psychology Medical sciences Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - metabolism NADPH Oxidases - genetics NADPH Oxidases - metabolism Rats Reactive Oxygen Species - metabolism Reverse Transcriptase Polymerase Chain Reaction Tetrazoles - pharmacology Vertebrates: cardiovascular system
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Zusammenfassung:	Asymmetrical dimethylarginine inhibits nitric oxide synthase, cationic amino acid transport, and endothelial function. Patients with cardiovascular risk factors often have endothelial dysfunction associated with increased plasma asymmetrical dimethylarginine and markers of reactive oxygen species. We tested the hypothesis that reactive oxygen species, generated by nicotinamide adenine dinucleotide phosphate oxidase, enhance cellular asymmetrical dimethylarginine. Incubation of rat preglomerular vascular smooth muscle cells with angiotensin II doubled the activity of nicotinamide adenine dinucleotide phosphate oxidase but decreased the activities of dimethylarginine dimethylaminohydrolase by 35% and of cationic amino acid transport by 20% and doubled cellular (but not medium) asymmetrical dimethylarginine concentrations (P
ISSN:	0194-911X 1524-4563
DOI:	10.1161/HYPERTENSIONAHA.110.152959