Efficacy of Lagenidium giganteum (Couch) metabolites for control Anopheles stephensi (Liston) a malaria vector

Background Lagenidium giganteum (Couch) a water mold of class Oomy-cetes, is a facultative parasite of mosquito. The metabolites of L. giganteum were tested for control of Anopheles stephensi. This fungus was grown in Peptone yeast extract glucose (PYG) broth in the laboratory at 25±2°C, and relative humidity was maintained at 75±5% for 15±2 days. The filtration process of metabolites was done using Whatman-1 filter paper and then with Flash chromatography. These purified metabolites were spray at five (3,4,5,6,7 ml/m2) different statistically significant concentrations. The efficacy after whatman-1 the LC50-5ml/m2, LC90-7.07 ml/m2 and LC99-10 ml/m2 values were found effective after exposure of 1 5h. LT50-10.23 h and LT90-16.59 h were recorded. Similarly, efficacy after Flash chromatography the LC50-4 ml/m2, LC90-6 ml/m2 and LC99-6.76ml/m2 values were found effective after exposure of 15h. The LT50-6.16h and LT90-12.02h were observed. This study reveals the metabolites of L. giganteum could be used for control of An. stephensi a malaria vector. Figures 1, 2, 3 Figure 1 figure1 The culture of L. giganteum in Peptone yeast extract glucose (PYG) broth in the laboratory. Full size image Figure 2 figure2 Probit regression line depicting relationship between probit of kill and log dose concentrations of metabolites of L. giganteum after 15 h exposure for An. stephensi. Full size image Figure 3 figure3 Probit regression line depicting relationship between probit of kill and log time of L. giganteum for An. stephensi. Full size image