Targeting DNA double-strand breaks with TAL effector nucleases

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and...

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Veröffentlicht in:	Genetics (Austin) 2010-10, Vol.186 (2), p.757-761
Hauptverfasser:	Christian, Michelle, Cermak, Tomas, Doyle, Erin L, Schmidt, Clarice, Zhang, Feng, Hummel, Aaron, Bogdanove, Adam J, Voytas, Daniel F
Format:	Artikel
Sprache:	eng
Schlagworte:	Arrays Binding Sites - genetics Catalytic Domain - genetics Deoxyribonucleases, Type II Site-Specific - chemistry Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism Deoxyribonucleic acid DNA DNA - genetics DNA Breaks, Double-Stranded DNA Repair DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Enzymes Gene Targeting Genetic Engineering Genetics Proteins Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcriptional Activation Xanthomonas Xanthomonas campestris Yeast Zinc Fingers - genetics
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Zusammenfassung:	Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
ISSN:	1943-2631 0016-6731 1943-2631
DOI:	10.1534/genetics.110.120717