Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins

Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 were used with MF59 adjuvant as a candidate vaccine for a phase 1 safety and immunogenicity trial. Ten of 41 vaccinee serum samples displayed a neutralization titer of ⩾1:20 against vesicular stomatitis virus (VSV)-HCV pseudotype, 15 of 36 ser...

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Veröffentlicht in:	The Journal of infectious diseases 2010-09, Vol.202 (6), p.862-866
Hauptverfasser:	Ray, Ranjit, Meyer, Keith, Banerjee, Arup, Basu, Arnab, Coates, Stephen, Abrignani, Sergio, Houghton, Michael, Frey, Sharon E., Belshe, Robert B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvants, Immunologic - administration & dosage Antibodies Antibodies, Neutralizing - blood Biological and medical sciences Cell culture techniques Epitopes Fundamental and applied biological sciences. Psychology Genetic Vectors Genotypes Glycoproteins Hepacivirus Hepacivirus - immunology Hepatitis C Antibodies - blood HIV - genetics Humans Infectious diseases Major and Brief Reports Medical sciences Microbiology Miscellaneous Placebos Polysorbates - administration & dosage Squalene - administration & dosage Vaccination Vesiculovirus - genetics Viral Envelope Proteins - immunology Viral Hepatitis Vaccines - immunology Virology Viruses Volunteerism
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Zusammenfassung:	Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 were used with MF59 adjuvant as a candidate vaccine for a phase 1 safety and immunogenicity trial. Ten of 41 vaccinee serum samples displayed a neutralization titer of ⩾1:20 against vesicular stomatitis virus (VSV)-HCV pseudotype, 15 of 36 serum samples tested had a neutralization titer of ⩾1:400 against human immunodeficiency virus (HIV)-HCV pseudotype, and 10 of 36 serum samples tested had a neutralization titer of ⩾1:20 against cell culture-grown HCV genotype 1a. Neutralizing serum samples had increased affinity levels and displayed >2-fold higher specific activity levels to well-characterized epitopes on E1/E2, especially to the hypervariable region 1 of E2.
ISSN:	0022-1899 1537-6613
DOI:	10.1086/655902