Preparation of Translationally Competent tRNA by Direct Chemical Acylation

Preparation of Translationally Competent tRNA by Direct Chemical Acylation

Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) us...

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Veröffentlicht in:Organic letters 2010-09, Vol.12 (17), p.3776-3779
Hauptverfasser: Duffy, Noah H, Dougherty, Dennis A
Format: Artikel
Sprache:eng
Schlagworte:
Acylation
Animals
Codon, Nonsense - genetics
Lanthanum - chemistry
Mice
Molecular Structure
Oocytes - metabolism
RNA, Transfer - chemical synthesis
RNA, Transfer - chemistry
RNA, Transfer, Amino Acyl - chemical synthesis
RNA, Transfer, Amino Acyl - chemistry
Xenopus - genetics
Xenopus - metabolism
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Zusammenfassung:Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol101408f