Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA
Abstract Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delive...
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| Veröffentlicht in: | Vaccine 2010-08, Vol.28 (36), p.5810-5816 |
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| creator | Torres-Escobar, Ascención Juárez-Rodríguez, María Dolores Branger, Christine G Curtiss, Roy |
| description | Abstract Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection. |
| doi_str_mv | 10.1016/j.vaccine.2010.06.070 |
| format | Article |
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Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.06.070</identifier><identifier>PMID: 20600475</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adhesins ; Allergy and Immunology ; Animals ; Antibiotics ; Antibodies, Bacterial - blood ; Antigens, Bacterial - immunology ; Applied microbiology ; Asd ; Bacterial Proteins - immunology ; Bacteriology ; Biological and medical sciences ; Codon-optimized ; Female ; Fundamental and applied biological sciences. Psychology ; Genomics ; Immune response ; Immunity, Humoral ; Immunity, Mucosal ; Immunization ; Immunoglobulin A - immunology ; Immunoglobulin G - blood ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mortality ; Plague ; Plague - prevention & control ; Plague Vaccine - immunology ; Plasmids ; Proteins ; PsaA antigen ; PsaB chaperone protein ; PsaC usher protein ; Salmonella enterica ; Salmonella enterica - immunology ; Studies ; Vaccine plasmid ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Attenuated - immunology ; Yersinia pestis - immunology</subject><ispartof>Vaccine, 2010-08, Vol.28 (36), p.5810-5816</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Elsevier Limited Aug 16, 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c611t-6517c31150c47f3a5d22bf9b1821c080fce33f86302624f890420ac778d1d6843</citedby><cites>FETCH-LOGICAL-c611t-6517c31150c47f3a5d22bf9b1821c080fce33f86302624f890420ac778d1d6843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1497401144?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27928,27929,45999,64389,64391,64393,72473</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23213620$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20600475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torres-Escobar, Ascención</creatorcontrib><creatorcontrib>Juárez-Rodríguez, María Dolores</creatorcontrib><creatorcontrib>Branger, Christine G</creatorcontrib><creatorcontrib>Curtiss, Roy</creatorcontrib><title>Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.</description><subject>Adhesins</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - immunology</subject><subject>Applied microbiology</subject><subject>Asd</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Codon-optimized</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genomics</subject><subject>Immune response</subject><subject>Immunity, Humoral</subject><subject>Immunity, Mucosal</subject><subject>Immunization</subject><subject>Immunoglobulin A - immunology</subject><subject>Immunoglobulin G - blood</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mortality</subject><subject>Plague</subject><subject>Plague - prevention & control</subject><subject>Plague Vaccine - immunology</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>PsaA antigen</subject><subject>PsaB chaperone protein</subject><subject>PsaC usher protein</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - immunology</subject><subject>Studies</subject><subject>Vaccine plasmid</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Yersinia pestis - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkl9rFDEUxQdR7Fr9CEpAxKddbzKZTOalUkr9AwWFKuhTyGbudLPOJNtkZmU_k1_SxF1b7UufAsnvnpyTnKJ4TmFBgYo368VWG2MdLhikPRALqOFBMaOyLuesovJhMQMm-JxT-HZUPIlxDQBVSZvHxREDAcDralb8Ot_qftKj9Y74jowrJKtp8EH3xA7D5JAEjBvvIhLryGANknzY78j-ej1iS37acUV6u82w8cMybbuR6HFEN_0BLnU_eId9rwm6EYM1mrSYJ4J1V0STiCZgJjsfhmzkO4ZondVkg3G0kXyO-vRp8ajTfcRnh_W4-Pru_MvZh_nFp_cfz04v5kZQOs5FRWtTUlqB4XVX6qplbNk1SyoZNSChM1iWnRRleh7GO9kAZ6BNXcuWtkLy8rg42etupuWArUmWU2S1CXbQYae8tur_E2dX6spvFWsY5aJJAq8PAsFfTymAGmw0Ob5DP0UloWaikgD3kjWXTQ2ykol8eYdc-ym49A6K8qbmQCnP1qs9ZYKPMWB345qCyr1Ra3Xojcq9USBU6k2ae_Fv5Jupv0VJwKsDoKPRfRe0MzbeciWjpWBZ6O2ew_RBW4tBRWPRGWxt6saoWm_vtXJyR8H0qQnp0h-4w3ibWkWmQF3mkueO01Rv2aTv-w3vpvuC</recordid><startdate>20100816</startdate><enddate>20100816</enddate><creator>Torres-Escobar, Ascención</creator><creator>Juárez-Rodríguez, María Dolores</creator><creator>Branger, Christine G</creator><creator>Curtiss, Roy</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20100816</creationdate><title>Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA</title><author>Torres-Escobar, Ascención ; Juárez-Rodríguez, María Dolores ; Branger, Christine G ; Curtiss, Roy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c611t-6517c31150c47f3a5d22bf9b1821c080fce33f86302624f890420ac778d1d6843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adhesins</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - immunology</topic><topic>Applied microbiology</topic><topic>Asd</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Codon-optimized</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torres-Escobar, Ascención</au><au>Juárez-Rodríguez, María Dolores</au><au>Branger, Christine G</au><au>Curtiss, Roy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2010-08-16</date><risdate>2010</risdate><volume>28</volume><issue>36</issue><spage>5810</spage><epage>5816</epage><pages>5810-5816</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>20600475</pmid><doi>10.1016/j.vaccine.2010.06.070</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesins Allergy and Immunology Animals Antibiotics Antibodies, Bacterial - blood Antigens, Bacterial - immunology Applied microbiology Asd Bacterial Proteins - immunology Bacteriology Biological and medical sciences Codon-optimized Female Fundamental and applied biological sciences. Psychology Genomics Immune response Immunity, Humoral Immunity, Mucosal Immunization Immunoglobulin A - immunology Immunoglobulin G - blood Mice Mice, Inbred BALB C Microbiology Miscellaneous Mortality Plague Plague - prevention & control Plague Vaccine - immunology Plasmids Proteins PsaA antigen PsaB chaperone protein PsaC usher protein Salmonella enterica Salmonella enterica - immunology Studies Vaccine plasmid Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Attenuated - immunology Yersinia pestis - immunology |
| title | Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA |
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