Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA

Abstract Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delive...

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Veröffentlicht in:	Vaccine 2010-08, Vol.28 (36), p.5810-5816
Hauptverfasser:	Torres-Escobar, Ascención, Juárez-Rodríguez, María Dolores, Branger, Christine G, Curtiss, Roy
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesins Allergy and Immunology Animals Antibiotics Antibodies, Bacterial - blood Antigens, Bacterial - immunology Applied microbiology Asd Bacterial Proteins - immunology Bacteriology Biological and medical sciences Codon-optimized Female Fundamental and applied biological sciences. Psychology Genomics Immune response Immunity, Humoral Immunity, Mucosal Immunization Immunoglobulin A - immunology Immunoglobulin G - blood Mice Mice, Inbred BALB C Microbiology Miscellaneous Mortality Plague Plague - prevention & control Plague Vaccine - immunology Plasmids Proteins PsaA antigen PsaB chaperone protein PsaC usher protein Salmonella enterica Salmonella enterica - immunology Studies Vaccine plasmid Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Attenuated - immunology Yersinia pestis - immunology
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Zusammenfassung:	Abstract Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2010.06.070