Turning the 10-23 DNAzyme On and Off with Light
Spaced out. DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self...
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| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2010-02, Vol.11 (3), p.320-324 |
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| container_title | Chembiochem : a European journal of chemical biology |
| container_volume | 11 |
| creator | Richards, Julia L Seward, Garry K Wang, Yu-Hsiu Dmochowski, Ivan J |
| description | Spaced out. DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self‐complementary strand via two photocleavable spacers remained “off” until UV irradiation restored activity. |
| doi_str_mv | 10.1002/cbic.200900702 |
| format | Article |
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| ispartof | Chembiochem : a European journal of chemical biology, 2010-02, Vol.11 (3), p.320-324 |
| issn | 1439-4227 1439-7633 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Base Sequence cage compounds DNA, Catalytic - chemistry DNA, Catalytic - metabolism DNA, Catalytic - radiation effects DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism DNA, Single-Stranded - radiation effects DNAzymes Kinetics light activation Nucleic Acid Denaturation oligonucleotides Photolysis photoregulation RNA - chemistry Ultraviolet Rays |
| title | Turning the 10-23 DNAzyme On and Off with Light |
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