Turning the 10-23 DNAzyme On and Off with Light

Spaced out. DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2010-02, Vol.11 (3), p.320-324
Hauptverfasser:	Richards, Julia L, Seward, Garry K, Wang, Yu-Hsiu, Dmochowski, Ivan J
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence cage compounds DNA, Catalytic - chemistry DNA, Catalytic - metabolism DNA, Catalytic - radiation effects DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism DNA, Single-Stranded - radiation effects DNAzymes Kinetics light activation Nucleic Acid Denaturation oligonucleotides Photolysis photoregulation RNA - chemistry Ultraviolet Rays
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Beschreibung
Zusammenfassung:	Spaced out. DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self‐complementary strand via two photocleavable spacers remained “off” until UV irradiation restored activity.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200900702