Aspergillus DNA contamination in blood collection tubes

Abstract Fungal polymerase chain reaction (PCR)-based diagnostic methods are at risk for contamination. Sample collection containers were investigated for fungal DNA contamination using real-time PCR assays. Up to 18% of blood collection tubes were contaminated with fungal DNA, probably Aspergillus...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2010-08, Vol.67 (4), p.392-394
Hauptverfasser:	Harrison, Elizabeth, Stalhberger, Thomas, Whelan, Ruth, Sugrue, Michele, Wingard, John R, Alexander, Barbara D, Follett, Sarah A, Bowyer, Paul, Denning, David W
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus Aspergillus - genetics Aspergillus fumigatus Blood - microbiology Blood collection tubes DNA DNA, Fungal - genetics DNA, Fungal - isolation & purification Humans Infectious Disease Internal Medicine Polymerase Chain Reaction Specimen Handling
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Beschreibung
Zusammenfassung:	Abstract Fungal polymerase chain reaction (PCR)-based diagnostic methods are at risk for contamination. Sample collection containers were investigated for fungal DNA contamination using real-time PCR assays. Up to 18% of blood collection tubes were contaminated with fungal DNA, probably Aspergillus fumigatus . Lower proportions of contamination in other vessels were observed.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2010.02.028