Selective isolation of Yersinia pestis from plague-infected fleas

We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48 h of growth using less selectiv...

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Veröffentlicht in:	Journal of microbiological methods 2010-07, Vol.82 (1), p.95-97
Hauptverfasser:	Sarovich, Derek S., Colman, Rebecca E., Price, Erin P., Chung, Wai Kwan, Lee, Judy, Schupp, James M., Cobble, Kacy R., Busch, Joseph D., Alexander, James, Keim, Paul, Wagner, David M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences CIN agar Culture Media - metabolism Fundamental and applied biological sciences. Psychology Insect Vectors - microbiology Isolation Microbiology Plague Plague - microbiology Selective Siphonaptera - microbiology Yersinia pestis Yersinia pestis - growth & development Yersinia pestis - isolation & purification Yersinia pestis - metabolism
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Beschreibung
Zusammenfassung:	We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pestis from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48 h of growth using less selective media.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2010.03.019