Fast-scanning high-flux microprobe for biological X-ray fluorescence microscopy and microXAS

There is a growing interest in the biomedical community in obtaining information concerning the distribution and local chemical environment of metals in tissues and cells. Recently, biological X‐ray fluorescence microscopy (XFM) has emerged as the tool of choice to address these questions. A fast‐sc...

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Veröffentlicht in:Journal of synchrotron radiation 2010-07, Vol.17 (4), p.522-529
Hauptverfasser: Barrea, R. A., Gore, D., Kujala, N., Karanfil, C., Kozyrenko, S., Heurich, R., Vukonich, M., Huang, R., Paunesku, T., Woloschak, G., Irving, T. C.
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Sprache:eng
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Zusammenfassung:There is a growing interest in the biomedical community in obtaining information concerning the distribution and local chemical environment of metals in tissues and cells. Recently, biological X‐ray fluorescence microscopy (XFM) has emerged as the tool of choice to address these questions. A fast‐scanning high‐flux X‐ray microprobe, built around a recently commissioned pair of 200 mm‐long Rh‐coated silicon Kirkpatrick–Baez mirrors, has been constructed at BioCAT beamline 18ID at the Advanced Photon Source. The new optical system delivers a flux of 1.3 × 1012 photons s−1 into a minimum focal spot size of ∼3–5 µm FWHM. A set of Si drift detectors and bent Laue crystal analyzers may be used in combination with standard ionization chambers for X‐ray fluorescence measurements. BioCAT's scanning software allows fast continuous scans to be performed while acquiring and storing full multichannel analyzer spectra per pixel on‐the‐fly with minimal overhead time (
ISSN:1600-5775
0909-0495
1600-5775
DOI:10.1107/S0909049510016869