Engineering CD19-specific T lymphocytes with interleukin-15 and a suicide gene to enhance their anti-lymphoma leukemia effects and safety

T lymphocytes expressing a chimeric antigen receptor (CAR) targeting the CD19 antigen (CAR.19) may be of value for the therapy of B-cell malignancies. Because the in vivo survival, expansion and anti-lymphoma activity of CAR.19 + T cells remain suboptimal even when the CAR contains a CD28 costimulatory endodomain, we generated a novel construct that also incorporates the interleukin-15 (IL-15) gene and an inducible caspase-9-based suicide gene (iC9/CAR.19/IL-15). We found that compared with CAR.19 + T cells, iC9/CAR.19/IL-15 + T cells had: (1) greater numeric expansion upon antigen stimulation (10-fold greater expansion in vitro , and 3- to 15-fold greater expansion in vivo ) and reduced cell death rate (Annexin-V + /7-AAD + cells 10±6% for iC9/CAR.19/IL-15 + T cells and 32±19% for CAR.19 + T cells); (2) reduced expression of the programmed death 1 (PD-1) receptor upon antigen stimulation (PD-1 + cells 40% for CAR.19 + T cells); and (3) improved antitumor effects in vivo (from 4.7- to 5.4-fold reduced tumor growth). In addition, iC9/CAR.19/IL-15 + T cells were efficiently eliminated upon pharmacologic activation of the suicide gene. In summary, this strategy safely increases the anti-lymphoma/leukemia effects of CAR.19-redirected T lymphocytes and may be a useful approach for treatment of patients with B-cell malignancies.