Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp

A normal prion protein (PrP∨c) is converted to a protease-resistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neuro-degenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cat...

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Veröffentlicht in:	Journal of veterinary science (Suwŏn-si, Korea) Korea), 2007-06, Vol.8 (2), p.131-137
Hauptverfasser:	Kang, S.G. (Seoul National University, Seoul, Republic of Korea), Lee, D.Y. (Seoul National University, Seoul, Republic of Korea), Kang, M.L. (Seoul National University, Seoul, Republic of Korea), Yoo, H.S. (Seoul National University, Seoul, Republic of Korea), E-mail: yoohs@snu.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis - physiology BSE Caspase 3 - metabolism Cattle Cell Growth Processes - physiology CHO Cells - cytology CHO Cells - enzymology CHO Cells - physiology CHO-K1 cells Cloning, Molecular Cricetinae Cricetulus Encephalopathy, Bovine Spongiform - genetics Encephalopathy, Bovine Spongiform - pathology Formazans Hydro-Lyases - metabolism Korean cattle Nitric Oxide - metabolism Original PRION PRIONES PRIONS Prions - biosynthesis Prions - genetics Prions - physiology Prnp Superoxide Dismutase - metabolism Tetrazolium Salts Transfection
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Beschreibung
Zusammenfassung:	A normal prion protein (PrP∨c) is converted to a protease-resistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neuro-degenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal antibody, 6H4. Cellular changes in the transfected CHO-K1 cells were investigated using parameters such as MTT, lactate dehydrogenase (LDH), and superoxide dismutase (SOD) activities, as well as nitric oxide (NO) production, and an apoptosis assay.
ISSN:	1229-845X 1976-555X
DOI:	10.4142/jvs.2007.8.2.131